Sequences from confirmation amplicon


Confirmation sequences for line 429B10 and hit At5g63580 are shown.
Line ID 429B10
Locus At5g63580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25455311
Primers used for PCR BH02 - 8474, show primer details
   
Sequence Name 23-K019175w-022-429-B10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K019175w-022-429-B10C-840
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATCATTGACAAACCACTCAAACCGAA
ATTTATCCGACTCATTTTATTTCCACTAAATTTGCTTATTTTCGGTACGTTTGTTCCTTT
CATATTCAACGTAAATAGTTAGATAAATAAACGATTCATTCTTTGGTATTTTTCCCCTAA
TTAACTAGACAGAAGACAACCATTTGTAGCCAAAGATTAGTAGTACCTGTATTGAGGAGG
ATTCTTGGGCCAGTAATCAAAATTGATACACGTCGGTGGCCAGATTCTGTGAAAAAGATT
CTCGGTCCAAACCTCACCTTTTACATACTTGAGATTCGTCGTGTAACCTTCGAAGTCTTT
GGAGCCATCTTGTTTTGCTACGGCTTTCTTCTCCGTCTCCGGGAGCTCGAAGAACTGTGT
ACCAACGTCCTTCAACCGCTGTATCAAATCCATCGGGATTCCGTGATTAACCACGTGAAA
AATGCCCCACTCTTCACTCCCTTTCACCACCGCATGTGCCACTAGCTCTTCGTCAAGATT
GCTTAG
   
Sequence Name 24-K019175w-022-429-B10C-BH02
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, BH02: 5'-CCCTATCATCGATCTAAGCAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K019175w-022-429-B10C-BH0
CTAGTGGCACATGCGGTGGTGAAAGGGAGTGAAGAGTGGGGCATTTTTCACGTGGTTAAT
CACGGAATCCCGATGGATTTGATACAGCGGTTGAAGGACGTTGGTACACAGTTCTTCGAG
CTCCCGGAGACGGAGAAGAAAGCCGTAGCAAAACAAGATGGCTCCAAAGACTTCGAAGGT
TACACGACGAATCTCAAGTATGTAAAAGGTGAGGTTTGGACCGAGAATCTTTTTCACAGA
ATCTGGCCACCGACGTGTATCAATTTTGATTACTGGCCCAAGAATCCTCCTCAATACAGG
TACTACTAATCTTTGGCTACAAATGGTTGTCTTCTGTCTAGTTAATTAGGGGAAAAATAC
CAAAGAATGAATCGTTTATTTATCTAACTATTTACGTTGAATATGAAAGGAACAAACGTA
CCGAAAATAAGCAAATTTAGTGGAAATAAAATGAGTCGGATAAATTTCGGTTTGAGTGGT
TTGTCAATGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11