Sequences from confirmation amplicon


Confirmation sequences for line 430C10 and hit At4g15765 are shown.
Line ID 430C10
Locus At4g15765 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8976611
Primers used for PCR PH85 - 8474, show primer details
   
Sequence Name 15-K021056-022-430-C10C-8409
GenBank Accession LN492771 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K021056-022-430-C10C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAGAAAAACGGAGAAA
AACGGAGAAGCTATCAAAGCAAAGGCACGTTTAACAACAGTAGATATATTTATTGATCAA
TTATTGAGTAGTGCCGTAGTGGAATTTTTTAGTATATACTTTATATTAGCCGACAGTGAC
ATTTATGGGTCGTGATCGGTTTTAATGGTTTGTGTTTGTTTGTTTTTCACTGAAAGCAAC
ATGTTTAGCATGAAATGCATGGTCTTATATATTTTGATAATATACTTTTCTTTTAATTTA
GGTTTTGATTGGCTGCGATGGGTCAAATTCTGTAGTCTCTAGATTTCTAGGATTAAACCC
GACTAAAGACCTCGGTTCGCGGGCCATCAGAGGGTTCACAAATTACCCGGACGACCATGG
ATTCCGACAAGAGTTTATAAGAATCAAGATGGACAACGTCGTAAGCGGACGACTTCCTAT
AACTCACAAACTCGTCTTTTGGTTTGTTGTTTTGCGAAATTGTCCCC
   
Sequence Name 16-K021056-022-430-C10C-PH85
GenBank Accession LN493241 [GenBank]
Sequencing primer gene specific primer, PH85: 5'-ATTCTTCACCATCTCTTTCCACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K021056-022-430-C10C-PH85
AAATTCGCGGACTGATGCTAGTGCCAATCTTGCGATATCCTCTTGATTTTTTAAGAAGTT
TGAGTCTATTTATAAAACAAATTATAAATACGTTAGTGCGTTATATGAAAACAATAGAAG
TCTATGTATAGTATGAAATGTAGCATGACCGGGTTATTAATTACCTTGGGGACAATTTCG
CAAAACAACAAACCAAAAGACGAGTTTGTGAGTTATAGGAAGTCGTCCGCTTACGACGTT
GTCCATCTTGATTCTTATAAACTCTTGTCGGAATCCATGGTCGTCCGGGTAATTTGTGAA
CCCTCTGATGGCCCGCGAACCGAGGTCTTTAGTCGGGTTTAATCCTAGAAATCTAGAGAC
TACAGAATTTGACCCATCGCAGCCAATCAAAACCTAAATTAAAAGAAAAGTATATTATCA
AAATATATAAGACCATGCATTTCATGCTAAACATGTTGCTTTCTGTGAAAAACAAACAAA
CACAAACCATTAAAACCGATCACGACCCATAAATGTCACTGTCGGCTAATATAAAGTATA
TACTAAAAAATTCCACTACGGCACTACTCAATAATTGATC

Last Updated on 06.12.2018 16:11