Sequences from confirmation amplicon


Confirmation sequences for line 430F05 and hit At3g14720 are shown.
Line ID 430F05
Locus At3g14720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4946538
Primers used for PCR 84zh - 8474, show primer details
   
Sequence Name 60-K125938-0022-430-F05C-84zh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 84zh: 5'-ATATCATTCCTCGTGACCTGTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K125938-0022-430-F05C-84zh
AAAAAAATTAGAATCCCCGGAAAATGAAAAAGAAATCCCTTTGCTTATAAAAACATTTCT
CATCTCCAGATTTTTTTTTCCCAACCCGTCTTTTCCCCAATCGCTGGATCTTAAAAATCT
CTAAATTTTCTCATCTCTCTCGGTTCACGAACTCTGGGTAAATAAAGTTCGCCTGTTTTC
CGAATTTCTGGAGAAAATCAGGGGTTTTTTCCTAAAACCCCAATCGGGTTTGGGTTTCGG
GGATTTTTTTTAAAAATTTGGGATCCAAAAAATGGCCTTTTCCTTTGATGAGCCAAATTT
TTAATTGGCAACCCCTCCATAGAAACCACCGAGGGGTTTTAAAATGAAATTTTTTAAAAT
TGACAAAAACAATAAATTTGTTGAGCGGATTTGTTTTTTTGGGGGGAAACCAATGAAGTT
AAATTTTGATTTAAAAAAGAGGCAAAAAACCCAGGAAAAAAAGGTGGGTGGCTTCTTTTT
CCCCGAAGGACCTTATAATTCCTTGGTCCTGGACAACCAATTTTGGAAAATTTATTGCCC
AAGGCTTTGTTCCAAAACAGAAAAGAAAGGGACTTTTCCCC
   
Sequence Name 57-K125937-0022-430-F05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K125937-0022-430-F05C-8409
GCATTTACATTGAATAATCCGGGTAAATTGGTAATTGCTCACGAGTCAAATCATCATTAG
CTTTGATGACTTGATGAAGATCTGATTCCATGAGCTCAAAAACAACATATATATCTTTAA
ACTCCCTCTTAGAAGGCGGAAGCATGATACTTTTGATTTCAACTATATCAGGGTGCCTAA
GAAGCCGTAGAAGCTTAACCTCACGGAGAATACGAAGAGCATCAGAGACATGCTCAAAAA
CATCATTAATCTTCTTAATAGCAACTTTCTCTCCTGTTTGTGTATCAATAGCTGCACATA
CAACTCCATAGCTTCCTTTCCCAATAACTTCAAGAATCCGATAACGATTAGCATCACCAT
ACTCAGTGAAAAACTCCATCTCTTTCATGTTCTGAAACATAGCATTAGCAAATAAATTAT
CAAAAATCGTATGTCAAGAACAAAGGAATCATAAGGTCCTCCGATGAATAAGAAGCAACC
AACCTTCTTCTCCTGAGTTTTTTGCATCCTCTTCAAATCAAATTTAAACTCTATCGTCTT
CACACCAAAAGATCAATACCGCTCAACAACTCTATCGTCTTCGTCAATTTCAAAAAATTC
AATTTAAAAACCCCTCGATGATCTCTATGTAGAGATCGCAATTAAAAAATATGACTAATC
AAAGAAAATGGCAATTCTTCGAATCACAAATTTTAAAAAAAAATCACAGAAACCAAAACC
CGATCGAGATTTAAGAAAAAAACCCTAGATTCTCACAAGAACTCCGAAAAACAGACGAAC
TCTATCTACACAGAGTCCGTGAACAGAGAGAGATGAGAAAATCTAGAGATCTTCAAGATC
CAGCGATCGGAGAAAAGACGGATAGGAAAATAAAAT

Last Updated on 06.12.2018 16:11