Sequences from confirmation amplicon


Confirmation sequences for line 431A04 and hit At2g18360 are shown.
Line ID 431A04
Locus At2g18360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7977194
Primers used for PCR BC18 - 8474, show primer details
   
Sequence Name 37-K103981-0022-431-A04C-BC18
GenBank Accession LN502592 [GenBank]
Sequencing primer gene specific primer, BC18: 5'-AGTGGATTCAATATGTCTTCGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K103981-0022-431-A04C-BC18
GGGCCGTAAGATATTAGGCCCTATAACTAGAAGATCCTCATTCTCTGATTCATAGAAAAT
CAAAATACTAGTGACATAGAATTGAACCATAGATTATTTTATGAAATATAATATATCAGG
TTACATTATTTTGAAAGAAGAACTGAAACCAAAGAAATAAGATAAATAAATTCATTCGGT
GTTGATGTGAGAATCGATTACATCCAAATTAGTTTTCGGAGTAAACTGAAGCAAGAAACT
TTTTGAGACGTCTGTTATAGACACAAGGTCTCTCCAAATGTGCCAAGTGACCTGCTTTTT
TTATACTCTCCATTGTTGCATTTTCGCCTAGTTGCCTTTAAAACAAAATATAAACGAGAC
TAAATTTATGTAATTAAAGAAGAAAAAAGTACTGTATTGAAACTTACTCTTTCATGCTCT
TGGCAAATTCAAGGTTAAAAATTTGATCACTTTCGCCCCACAGAAGATGTATTTTCTGCC
AAACCAAAAAAGAATAATAATATCAATTGATGCGTTAAGTAGTAAATTTTAATTATATTT
TAGATTTAAGTTGTAATATCAGCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTC
   
Sequence Name 53-K103982-0022-431-A04C-8409
GenBank Accession LN508135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K103982-0022-431-A04C-8409
GCCATTGCTGATATTACAACTTAAATCTAAAATATAATTAAAATTTACTACTTAACGCAT
CAATTGATATTATTATTCTTTTTTGGTTTGGCAGAAAATACATCTTCTGTGGGGCGAAAG
TGATCAAATTTTTAACCTTGAATTTGCCAAGAGCATGAAAGAGTAAGTTTCAATACAGTA
CTTTTTTCTTCTTTAATTACATAAATTTAGTCTCGTTTATATTTTGTTTTAAAGGCAACT
AGGCGAAAATGCAACAATGGAGAGTATAAAAAAAGCAGGTCACTTGGCACATTTGGAGAG
ACCTTGTGTCTATAACAGACGTCTCAAAAAGTTTCTTGCTTCAGTTTACTCCGAAAACTA
ATTTGGATGTAATCGATTCTCACATCAACACCGAATGAATTTATTTATCTTATTTCTTTG
GTTTCAGTTCTTCTTTCAAAATAATGTAACCTGATATATTATATTTCATAAAATAATCTA
TGGTTCAATTCTATGTCACTAGTATTTTGATTTTCTATGAATCAGAGAATGAGGATCTTC
TAGTTATAGGGCCTAATATCTATACGGCCCAATTACAAAAGTGTCAATGGGTCCGAAGAC

Last Updated on 06.12.2018 16:11