Sequences from confirmation amplicon


Confirmation sequences for line 431C10 and hit At2g37040 are shown.
Line ID 431C10
Locus At2g37040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15558842
Primers used for PCR PE19 - 8474, show primer details
   
Sequence Name 31-K022407-022-431-C10C-8409
GenBank Accession LN499981 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K022407-022-431-C10C-8409
TGATCCAGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTCACCACTCA
GTAGATGATCGGTGAACTCAGGCTTACCACTCATCACCTCTGCGAAAACCGCCGACAAAA
TCTCAGCCAAAACAGAGAGAACATTCGTTTCGAATAACACCATTGACGCCATTCCAGATC
CAACCGCCGTGCCATTGACTAGCGCGAGACCTTCCTTAGGCTGGAGATCAAAGAATCCGG
AGCTGATTCCTGCTAATTTGAAAGCTTCCTCTGCTGTTAAAGCTTCACCGTTGGGACCAG
TAGCTTTGGAATTGGGACGACCGGTGAGAAGTCCGGCGATGTAGGAGAGAGGAACGAGAT
CTCCGGAGGCGGTGATTGTACCACGGAGGGGGAGAGATGGAGTGATGTTG
   
Sequence Name 32-K022407-022-431-C10C-PE19
GenBank Accession LN500408 [GenBank]
Sequencing primer gene specific primer, PE19: 5'-CTCAACAACAACATCACTCCATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K022407-022-431-C10C-PE19
CCCCTCCGTGGTACAATCACCGCCTCCGGAGATCTCGTTCCTCTCTCCTACATCGCCGGA
CTTCTCACCGGTCGTCCCAATTCCAAAGCTACTGGTCCCAACGGTGAAGCTTTAACAGCA
GAGGAAGCTTTCAAATTAGCAGGAATCAGCTCCGGATTCTTTGATCTCCAGCCTAAGGAA
GGTCTCGCGCTAGTCAATGGCACGGCGGTTGGATCTGGAATGGCGTCAATGGTGTTATTC
GAAACGAATGTTCTCTCTGTTTTGGCTGAGATTTTGTCGGCGGTTTTCGCAGAGGTGATG
AGTGGTAAGCCTGAGTTCACCGATCATCTACTGAGTGGTGAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11