Sequences from confirmation amplicon


Confirmation sequences for line 431G08 and hit At5g36940 are shown.
Line ID 431G08
Locus At5g36940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14591193
Primers used for PCR 56ra - 8474, show primer details
   
Sequence Name 32-K115914-0022-431-G08C-56ra
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 56ra: 5'-ACCCCGTAAGGAAAATACCTAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K115914-0022-431-G08C-56ra
TGAATCACTTATGAGTAAAATGGACAATAAGGAGAAAATGAACGAGTTATTACCAATTTT
TTTTCACATTACACTTTGTAT
   
Sequence Name 06-K115913-0022-431-G08C-8409
GenBank Accession LN488957 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K115913-0022-431-G08C-8409
TTTTTCTTTACTAAAGTGTGGTTATATATTGTTTTGCTGAACTGCAGTTTAGAAGTGTTG
CGTGTTTAGAACTGGGTAGGTAGGGATATCCACATAGCTCTATAAGATTTACGCTCTTGT
GTTGTTTGTGCAATGACTCTATTTTTAGGATTCTTGCTAATAAATATTGAGTGGCACTTA
GAGATTGAAAGAAAAAGGCGACATTTCTTTTTGCAAAGCTTTGGATATCTTTGTGTTCAC
CAATTCGTTTGGTTTTTCTTGTTTTTAACATGAACATGTGGAAATTGTTTTGTTTATCAT
GTGATGACATTTTAATATAACATGAATAGGCAATGATTTTCGGTGGTGAAGATTGTCTGC
CTACAATATTAGCTCGTCATCAAATCCCAGGCCTTGATATTGTTGTTGACCCGTGTGCTG
CTGTGCTAGTGTTCATTGTAACAGGACTTTGTTGCCTTGGAGTAAAGGAGGTATTGATTT
CATATTATTTCAATTAAATCGTTTGGACAGACTCAACTCTGCCGAGCTGTGTTTTCTTAA
AGTGAAATATCTCTTCTCTGCAGAGCACATTTGCTCAGGGAATTGTAACAACAGCAAATG
TCTTTGTCATGATATTTGTCATAGTAGCTGGAAGCTATTTGTGTTTCAAGACAGGTTGGG
TTGGTTATGAACTTCCAACGGGGTAAACTGAACGTTCCTTGTGCTACCACTATCATATTG
TAAGGTAGAAGCTATATGAATGTTTTTTTCTTATTGTTTCTTAGGTATTC

Last Updated on 06.12.2018 16:11