Sequences from confirmation amplicon


Confirmation sequences for line 431G10 and hit At4g00900 are shown.
Line ID 431G10
Locus At4g00900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:383039
Primers used for PCR 32bk - 8474, show primer details
   
Sequence Name 50-K107839-0022-431-G10C-32bk
GenBank Accession LN507189 [GenBank]
Sequencing primer gene specific primer, 32bk: 5'-ACTGTGTCTTTGGTTGAGGTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K107839-0022-431-G10C-32bk
TGTAGAAAAGTACTGTCTTTTGTCTTTTGTGTTGATCAGACTTTTACTTGTCTTGATCTT
CTTCTTGATTCTTGTCCTTCTGCATCTCCCAATGAACTTGAGAGCTTCATCGATCAGAAT
CACAGGGAAGGAAACAAGAATAACCACAAACCATTCCCTGAAACTCAAAGGCACAATCCC
AAACACATTAGCTAAGAAAGGAACATAGAGGATGACACAGTGTAGCGCAAAGGAGACAGT
CATGGCCACTAGTAGCCAAGGGTTTCTCCATGGTGGCATCGTTAAGAGACTGTTGTCTTC
AGACAACGCGTTCAGCGAGTTAAACATTTCGATAGCCACAAGAACGGTGAGTGATAATGT
CATTGGCTTGACTTTCCCGAGAGTGAAATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 82-K107840-0022-431-G10C-8409
GenBank Accession LN514201 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K107840-0022-431-G10C-8409
TTACATTGAATTATTTCACTCTCGGGAAAGTCAAGCCAATGACATTATCACTCACCGTTC
TTGTGGCTATCGAAATGTTTAACTCGCTGAACGCGTTGTCTGAAGACAACAGTCTCTTAA
CGATGCCACCATGGAGAAACCCTTGGCTACTAGTGGCCATGACTGTCTCCTTTGCGCTAC
ACTGTGTCATCCTCTATGTTCCTTTCTTAGCTAATGTGTTTGGGATTGTGCCTTTGAGTT
TCAGGGAATGGTTTGTGGTTATTCTTGTTTCCTTCCCTGTGATTCTGATCGATGAAGCTC
TCAAGTTCATTGGGAGATGCAGAAGGACAAGAATCAAGAAGAAGATCAAGACAATGTAAA
AGTCTGATCAACACAAAAGACAAAAGACAGTAACTTTTTTCTTACAATCTTTTGTTTCAT
TAAGAAACCTCAACCAAAGACACAG

Last Updated on 06.12.2018 16:11