Sequences from confirmation amplicon


Confirmation sequences for line 431H06 and hit At5g03300 are shown.
Line ID 431H06
Locus At5g03300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:796591
Primers used for PCR 39sj - 8474, show primer details
   
Sequence Name 64-K116453-0022-431-H06C-39sj
GenBank Accession LN511278 [GenBank]
Sequencing primer gene specific primer, 39sj: 5'-TTTAACCCATCAAATACCAAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K116453-0022-431-H06C-39sj
GGCATGTTTGTCTTCGCGAGATCGCATTGTTCAACTTGATGTCGTATCTGTCAACACGAT
TAGAGAACAACATAAACAAAAAAAACGCACACAAATCACAGAGTTTATGCCACTGAAGAA
GAATCAAACTATCAAATCTAGATTCAAACCAAAATCCCAGAGAGAGATAGCGACAATGGA
TACAGCAAAAGGAATTGGAAACAAATAGAAAAAGAGGGATCTGAGAGTTTATTTACTTGG
TGAGAAATTCGTCGTCGACGACGGCAGAGATATCGAGGAGTGGGTTACCCATACCGAGAA
GGATTCCATCGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCACAGTTTATTA
   
Sequence Name 31-K116452-0022-431-H06C-8409
GenBank Accession LN500308 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K116452-0022-431-H06C-8409
ATTTACGATGGATCCTTCTCGGTATGGGTAACCCACTCCTCGATATCTCTGCCGTCGTCG
ACGACGAATTTCTCACCAAGTAAATAAACTCTCAGATCCCTCTTTTTCTATTTGTTTCCA
ATTCCTTTTGCTGTATCCATTGTCGCTATCTCTCTCTGGGATTTTGGTTTGAATCTAGAT
TTGATAGTTTGATTCTTCTTCAGTGGCATAAACTCTGTGATTTGTGTGCGTTTTTTTTGT
TTATGTTGTTCTCTAATCGTGTTGACAGATACGACATCAAGTTGAACAATGCGATTCTCG
CGGAAGACAAACATTTGCCCATGTAAGTATTGATTTATGCTCTGCTTTTGGTATTGATGG
GGTTAAAA

Last Updated on 06.12.2018 16:11