Sequences from confirmation amplicon


Confirmation sequences for line 432D08 and hit At5g28050 are shown.
Line ID 432D08
Locus At5g28050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:10044967
Primers used for PCR 01bc - 8474, show primer details
   
Sequence Name 46-K107114-0022-432-D08C-8409
GenBank Accession LN505876 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K107114-0022-432-D08C-8409
TATTTTTGATTTATGTGTCCCCATTCTTAGTTGAAGATTGTATGTGTTTGCAGAAGTTGT
TGTGGTTTATGTTTCTATACTTTGTCTTTGATCAGCTGTTCACGATAGCGACCATAAATT
CCTAACGCAAGCTGTTGAAGAAGCTTACAAGGGAGTGGACTGTGGTGATGGTGGCCCATT
TGGTGCGGTGATTGTGCATAATAACGAGGTTGTCGCTAGCTGCCACAATATGGTTTTGAA
ATATACTGACCCAACTGCACATGCTGAAGTCACTGCCATTAGAGAGGTAAGAAAAGAGTA
TTACGCTTGAATCTCTTGAAACGTTGCAAAAACCGGTTTTCTGATGATGATTTAATTCCA
TTG
   
Sequence Name 62-K107115-0022-432-D08C-01bc
GenBank Accession LN510770 [GenBank]
Sequencing primer gene specific primer, 01bc: 5'-ACAATGGATTCAAATCATCATCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K107115-0022-432-D08C-01bc
AGCGTAATACTCTTTTCTTACCTCTCTAATGGCAGTGACTTCAGCATGTGCAGTTGGGTC
AGTATATTTCAAAACCATATTGTGGCAGCTAGCGACAACCTCGTTATTATGCACAATCAC
CGCACCAAATGGGCCACCATCACCACAGTCCACTCCCTTGTAAGCTTCTTCAACAGCTTG
CGTTAGGAATTTATGGTCGCTATCGTGAACAGCTGATCAAAGACAAAGTATAGAAACATA
AACCACAACAACTTCTGCAAACACATACAATCTTCAACTAAGAATGGTGGACACATAAAT
CAAAAATAGCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCACACGATTTATT

Last Updated on 06.12.2018 16:11