Sequences from confirmation amplicon


Confirmation sequences for line 432E09 and hit At1g65500 are shown.
Line ID 432E09
Locus At1g65500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24358115
Primers used for PCR 24op - 8474, show primer details
   
Sequence Name 31-K114909-0022-432-E09C-24op
GenBank Accession LN500280 [GenBank]
Sequencing primer gene specific primer, 24op: 5'-AACCACTGATCATCAAAAGGATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114909-0022-432-E09C-24op
TATTTTTCGTCTTAATTAAACACAATACTTTTTGTTTACATCATTTGAAAGGAGCACATG
TTTTTTAGATGTGTAAACCAAATCTTAAAACCACATACACTTTATTATTGCATAGAACTT
CAAAACTCTGACACAAAGACACAGAAGTACGTAGTTAAGGAGATTGGTCAGAAGCAGGAA
TATAACTGCCTGGAGGAAGCCTGCATGGTCGGGGTCTCCTCCACTTCGAAGTGAATACTG
CTGGAGAAGTTCCACATGACCTTGAAGGTGGTGTGTACACGATAGGTGTTCCCGAAAACC
CCCACGCTGCCNTATTAAAGTTCAAAACTAAAATAAGGGAGATTTTTAATTTACTTTGGT
GGAAAAATAGATTTCCAGCTCGTTCTTTTCACAACTTTATTTTAGAAATTGTTGATGCAA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATTCC
   
Sequence Name 07-K114908-0022-432-E09C-8409
GenBank Accession LN489391 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114908-0022-432-E09C-8409
CATTCAACAATTTCCTAAAATAAAGTTGTGAAAAGAACGAGCTGGAAATCTATTTTTCCA
CCAAAGTAAATTAAAAATCTCCCTTATTTTAGTTTTGAACTTTAATATGGCAGCGTGGGG
GTTTTCGGGAACACCTATCGTGTACACACCACCTTCAAGGTCATGTGGAACTTCTCCAGC
AGTATTCACTTCGAAGTGGAGGAGACCCCGACCATGCAGGCTTCCTCCAGGCAGTTATAT
TCCTGCTTCTGACCAATCTCCTTAACTACGTACTTCTGTGTCTTTGTGTCAGAGTTTTGA
AGTTCTATGCAATAATAAAGTGTATGTGGTTTTAAGATTTGGTTTACACATCTAAAAACA
TGTGCTCCTTTCAAATGATGTAAACAAAAAGTATTGTGTTTAATTAAAACTAAAAATAAT
ATTGTTGATCATAAATCATAATATCGAAATAGTTGTTGAATCCTTTT

Last Updated on 06.12.2018 16:11