Sequences from confirmation amplicon


Confirmation sequences for line 432F04 and hit At3g25230 are shown.
Line ID 432F04
Locus At3g25230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9191051
Primers used for PCR 16hg - 8474, show primer details
   
Sequence Name 67-K112758-0022-432-F04C-16hg
GenBank Accession LN511845 [GenBank]
Sequencing primer gene specific primer, 16hg: 5'-TCCTTCAGTGAGGAAGAGAAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K112758-0022-432-F04C-16hg
ATCTAACGATGCGCCTGCAACTCAATTGAAAGATTACAGCAGGCCGAAAAACTCTTACAA
AGGTAATAATAATTGGTTGTTAAGACCACAAGTCCTTTATGTCAGTTTTAAAGCCTAGCT
GAACAAAACTGATGGAGATGGTGGTTTTGGTATTTAGGTGTTAGAGCTAGAAAGCACCAA
TGTGAAGGCCTTATACCGGAGAGCGCAAGCGTACATGGAGTTGTCTGATTTAGACTTGGC
CGAGTTTGATGTCAAGAAGGCCCTTGAAATCGATCCCAACAACAGGTTTGTCTCTTACTC
TTTAACTCTTATTTAATGTTACATTTGGCTTTTGTATCTTTTAATCCACAGCATGATAAT
GTCTTCTTTATATACTAACAGAAGACTAAATTTGCTATTTTATTTGCTAATGATAGGGAA
GTGAAGCTAGAGCAGAAAAGGTTGAAGGAGAAAATGAAGGAATTCAATAAAAAGGAAGCC
AAATTTTATGGAAACATGTTTGCGAAACTAAGTGTAAGTATCTCAATGGTTTGAATTGTA
TGTAGATACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCG
   
Sequence Name 27-K112757-0022-432-F04C-8409
GenBank Accession LN498463 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K112757-0022-432-F04C-8409
GCATTTCATTGAATATATCCTGTATCTACATACAATTCAAACCATTGAGATACTTACACT
TAGTTTCGCAAACATGTTTCCATAAAATTTGGCTTCCTTTTTATTGAATTCCTTCATTTT
CTCCTTCAACCTTTTCTGCTCTAGCTTCACTTCCCTATCATTAGCAAATAAAATAGCAAA
TTTAGTCTTCTGTTAGTATATAAAGAAGACATTATCATGCTGTGGATTAAAAGATACAAA
AGCCAAATGTAACATTAAATAAGAGTTAAAGAGTAAGAGACAAACCTGTTGTTGGGATCG
ATTTCAAGGGCCTTCTTGACATCAAACTCGGCCAAGTCTAAATCAGACAACTCCATGTAC
GCTTGCGCTCTCCGGTATAAGGCCTTCACATTGGTGCTTTCTAGCTCTAACACCTAAATA
CCAAAACCACCATCTCCATCAGTTTTGTTCAGCTAGGCTTTAAAACTGACATAAAGGACT
TGTGGTCTTAACAACCAATTATTATTACCTTTGTGCATAGTTTTTCGGCCTGCTTGTAAT
CTTTCAATTTGAGTTTGCAGGCTGCATCGTTTAGATTGCATGCCACCTTCAATGCTTTTG
CTTGCTTCTTCTCTT

Last Updated on 06.12.2018 16:11