Sequences from confirmation amplicon


Confirmation sequences for line 434G06 and hit At5g38160 are shown.
Line ID 434G06
Locus At5g38160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15225984
Primers used for PCR 52rn - 8474, show primer details
   
Sequence Name 30-K116083-0022-434-G06C-52rn
GenBank Accession LN499863 [GenBank]
Sequencing primer gene specific primer, 52rn: 5'-TTTATCTTAGTTAACGCAGCCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K116083-0022-434-G06C-52rn
GACCCACTAGATATTTATTCTTAAATACAGCTTATACAACTAGGTCCAAAAAATTATACA
TATACTATAACGGACGTACCACATACTTAACATGTGTTTGAGCAATTGTGAATCATGATT
ATTTTCACGTCTTTATGCATGATTGCCAAATGTATGAACGTTAATTAATAAGACAAGACC
TAAAGGCTTGTCCGTTACAACTACATCTACTATATATAACAATAGTTCTACCATCTTGAA
AATCACAAAACACATTTTCAAATATACTTCAAAGAAAAAAAAAAATAAAGCTGTGAAAAG
GATTTTCAAAACATGAATTTCACGGGAGTAGTGTTCATTTTGTTCGTGTTAGGTACCATG
TTGTCTCCAGTTCCAGTCAAAGCAAGGGTGGTCAAAGGTTCCGGAAAAAAAGTGAACGTA
ACGTGTGATGCAACCCACCTAATTTCATGTGTAACACCAGTGAAAAGGCGCATAAAACTA
CTTGTCCGGAAAATCTACTTGGATCACCAATGATTATGATGGTCAATATGGAGAAAAAAA
AAAAGTAATTACCAATTTTTTTTCATTTCAAAAATGTAAATTTCCGCACCGTTATAAAAA
AAAAA
   
Sequence Name 14-K116082-0022-434-G06C-8409
GenBank Accession LN492656 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K116082-0022-434-G06C-8409
CGCCTTTTCACTGCTGTTACACATGAACTAAGCTGCGTCGCATCACACGTTACGTTCACT
TCTTCTCCGGAACCTTTGACCACCCTTGCTTTGACTGGAACTGGAGACAACATGGTACCT
AACACGAACAAAATGAACACTACTCCCGTGAACTTCATGTCTTGAAAATCCTTTTCACAG
CTTTATTTTTTTTTTTCTTTGAAGTATATTTAAAAATGTGTTTTGTGATTTTCAAAATGG
TAAAACTATGGTTATATATAGTAAATGTAGTTGTAACGGACAACCCTTTAGGTCTTGTCT
TATTAATTAACGTTCATACATTTGGCAATCATGCATAAAGACGTGAAAATAATCATGATT
CACAATTGCTCAAACACATGTTAAGTATGTGGTACGTCCGTTATAGTATATGTATAATTT
TTTGGACCTAGTTGTATAACCTGTATTTAAAAATAAATATCAGGTGGGTCTATAAAAAAG
TTAAAAAAAAGTTTCATACGTGTTAAGTTTGTTTACGTCCGTTAAAGTAAATAATTTATT
GGGCCTAATATGGGCTGCGTTAACTAAAATAAAA

Last Updated on 06.12.2018 16:11