Sequences from confirmation amplicon


Confirmation sequences for line 435A12 and hit At1g74300 are shown.
Line ID 435A12
Locus At1g74300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27935396
Primers used for PCR 34tv - 8474, show primer details
   
Sequence Name 51-K117029-0022-435-A12C-34tv
GenBank Accession LN507617 [GenBank]
Sequencing primer gene specific primer, 34tv: 5'-TGAACCTTAGTTCCAAGAGTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K117029-0022-435-A12C-34tv
TTTGTGTTTCGCTTTCTCTCTTGGAAGTCCATACTTCTTGTATGCCAAACGTCTCCCGTC
TCGCAGCTTTATACGCGGTGGCTGTGATCGGTGGACCACCGGGGGAGCCACATAGATTCG
GTGGAGGAGGTTTCAAGATTGACTGATAAGTCAACGCCAAGATTATCCCAACAATAATGA
TCACACTTGATGGTCCAATCGTGGAAATGGCGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 19-K117028-0022-435-A12C-8409
GenBank Accession LN494928 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117028-0022-435-A12C-8409
TTATTGTTGGGATAATCTTTGGCGTTGACTTATCAGTCAATCTTGAAACCTCCTCCACCG
AATCTATGTGGCTCCCCCCGGTGGTCCACCGATCACAGCACCGCGTATAAAGCTGCGAGA
CGGGAGACATTTGGCATACAAGGAGTATGGACTTCCAAGAGAGAAAGCTAAACACAAAAT
AGTCTTCATCCATGGATCCGATAGTTGTAGACACGACGCCGTTTTCGCCACTCTTCTTTC
TCCGGTACGTTCTTACCAACTCTTGACTTTTAAGGTTC

Last Updated on 06.12.2018 16:11