Sequences from confirmation amplicon


Confirmation sequences for line 435H06 and hit At3g13390 are shown.
Line ID 435H06
Locus At3g13390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4351964
Primers used for PCR CC21 - 8474, show primer details
   
Sequence Name 45-K106187-0022-435-H06C-CC21
GenBank Accession LN505538 [GenBank]
Sequencing primer gene specific primer, CC21: 5'-TCTCTTTACCTTGAAACCTGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K106187-0022-435-H06C-CC21
GTACGTGGGTCTCAGACATCTCTAACTTTAGGATTCAGAATCACAAGTTGAAGCTTGTTG
AGATGGAAGGATCGCACGTTCTCCAGAACGATTATGACTCTCTCGACGTTCACGTTGGTC
AGTGTTACGGAACCATCCTTACCGCTAATCAGGAAGCTAAAGATTACTACATGGTTGCAT
CCTCTAGGTTCTTGAAGTCGGTTATAACCACCACTGGTCTTCTCCGCTATGAGGGAGGCA
AAGGTCCCGCTTCTTCGCAGCTACCCCCTGGTCCAGTCGGCTGGGCTTGGTCCTTGAACC
AGTTCCGATCTTTCAGGTGGAACTTGACCGCCAGTGCAGCCAGGCCTAACCCTCAGGGAT
CTTACCACTACGGAAAGATCAACATCACACGCACCATCAAGCTTGTGAACACTCAAGGCA
AGGTCGATGGAAAGCTCAGGTACGCTTTGAACGGAGTCTCGCACACTGACCCCGAAACCC
CATTGAAGCTTGCGGAGTACTTTGGTGTTGCCGACAAGGTTTTCAAGTACGACTCCATCA
CTGATAACCCAACCCCAGAACAAATCAAGAGCATCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCG
   
Sequence Name 29-K106188-0022-435-H06C-8409
GenBank Accession LN499298 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K106188-0022-435-H06C-8409
CTCTTGATTTGTTCTGGGGTTGGGTTATCAGTGATGGAGTCGTACTCCAATAACTTGTCG
GCAACACCAAAGTACTCCGCAAGCTTCAATGGGGTTTCGGGGTCAGTGTGCGAGACTCCG
TTCAAAGCGTACCTGAGCTTTCCATCGACCTTGCCTTGAGTGTTCACAAGCTTGATGGTG
CGTGTGATGTTGATCTTTCCGTAGTGGTAAGATCCCTGAGGGTTAGGCCTGGCTGCACTG
GCGGTCAAGTTCCACCTGAAAGATCGGAACTGGTTCAAGGACCAAGCCCAGCCGACTGGA
CCAGGGGGTAGCTGCGAAGAAGCGGGACCTTTGCCTCCCTCATAGCGGAGAAGACCAGTG
GTGGTTATAACCGACTTCAAGAACCTAGAGGATGCAACCATGTAGTAATCTTTAGCTTCC
TGATTAGCGGTAAGGATGGTTCCGTAACACTGACCAACGTGAACGTCGAGAGAGTCATAA
TCGTTCTGGAGAACGTGCGATCCTTCCATCTCAACAAGCTTCAACTTGTGATTCTGAATC
CTAAAGTTAAGAGATGTCTTGAGACCCACGTTACAGATCCTAACCCTGTAGGTCTTTCCA
GGTTTCAGGNTTAAAGAGA

Last Updated on 06.12.2018 16:11