Sequences from confirmation amplicon


Confirmation sequences for line 436B01 and hit At1g26665 are shown.
Line ID 436B01
Locus At1g26665 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9214585
Primers used for PCR CC22 - 8474, show primer details
   
Sequence Name 11-K106284-0022-436-B01C-8409
GenBank Accession LN491122 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K106284-0022-436-B01C-8409
GGAATGAGCCATTACTTCAACTGTGACCGTTGCTGATGATTCAAAGGAGAATCTTTCCCA
AGTCATAAACTCTATTGAGAAAACTTTAGGAGTCCTTCATCAGTTACACCTTACCGTCAC
TTCTTTCACACCCGCGTCTCAACTCCATCTCCTCCAGCGCCTGTAAGTAACTCCTCTACT
CATCTGTGTTTATTGTTTTGGGTATTGTAACTATTGTTTGAATGAATATGTGTGTGTGTG
TGTATTTGCAGTAACTCTCTTGTGATGGAGCTAGATAACATGACTAAGTTGTCTGAGAAA
TGCAACATTCAAATCCCCATGGAGGTTCTTAAGTGAGAAAACAAGTCCTTTTTGTTTTTA
GTATGCACTTTGAAGATAGATACTTTTACTTGCTTACTTATGTTTGCGTTTTCGTATAGC
TTGATTGATGATGGGAAGAACCCGGATGAGTTTACGAAAGATGTTCTTAATAGCTGCATA
GCTAGAAATCAAGTTACTAAGGGCAAGACTGATGCTTTTAAGGTGACTCC
   
Sequence Name 11-K106285-0022-436-B01C-CC22
GenBank Accession LN491123 [GenBank]
Sequencing primer gene specific primer, CC22: 5'-AAAGGAGTCACCTTAAAAGCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K106285-0022-436-B01C-CC22
GCAGCTATAAGAACATCTTTCGTAAACTCATCCGGGTTCTTCCCATCATCAATCAAGCTA
TACGAAAACGCAAACATAAGTAAGCAAGTAAAAGTATCTATCTTCAAAGTGCATACTAAA
AACAAAAAGGACTTGTTTTCTCACTTAAGAACCTCCATGGGGATTTGAATGTTGCATTTC
TCAGACAACTTAGTCATGTTATCTAGCTCCATCACAAGAGAGTTACTGCAAATACACACA
CACACACATATTCATTCAAACAATAGTTACAATACCCAAAACAATAAACACAGATGAGTA
GAGGAGTTACTTACAGGCGCTGGAGGAGATGGAGTTGAGACGCGGGTGTGAAAGAAGTGA
CGGTAAGGTGTAACTGATGAAGGACTCCTAAAGTTTTCTCAATAGAGTTTATGACTTGGG
AAAGATTCTCCTTTGAATCATCAGCAACGGTCACAGTTGAAGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11