Sequences from confirmation amplicon


Confirmation sequences for line 436D05 and hit At1g09560 are shown.
Line ID 436D05
Locus At1g09560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3094211
Primers used for PCR CM52 - 8474, show primer details
   
Sequence Name 09-K104123-0022-436-D05C-CM52
GenBank Accession LN490151 [GenBank]
Sequencing primer gene specific primer, CM52: 5'-AGCTCTGATCCATCCGTTTTAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K104123-0022-436-D05C-CM52
TTAGACAGATGTGTGCAAGAAAGAAAAACAGCATGTTGCTTGCTTTTTCCCTCAACGAGA
AAAGTCACCCTCTCTTCAATTCTTCAATTCTCCTCCCTTTATAAACCATAATTCATTAAT
TCTCTTCTCTCTCAACAACTACTTGTTTAGAGAGAGTCCCACCACCACCACTACCACAAT
CCCAATGGCTTCACCAACTCTTACTCTCCTCCTCCTCCTCACCACCGTCTCCTTCTTCAT
CTCCTCCTCCGCAGATCCTGACATGCTTCAAGATCTCTGCGTCGCTGATCTTCCCTCTGG
TACTTGCAAAAACCTTTACTTTCATCTCTTTTCCTAGAAAAAGATTCAATTTCTAATCTC
TTTCTTGTTTTTCTTTTTTGCAGGAATCAAAATCAATGGCTTCCCTTGCAAAGACGCAGC
TACAGTCACATCAGCCGATTTCTTCTCACAAGGATTAGCTAAACCAGGTCTTACCAACAA
CACATTCGGTGCCTTAGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAAAGTAGATATCCGCACGTTTTTT
   
Sequence Name 25-K104124-0022-436-D05C-8409
GenBank Accession LN497415 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K104124-0022-436-D05C-8409
TGAGCCATTTACAATTGACTAAGGCACCGAATGTGTTGTTGGTAAGACCTGGTTTAGCTA
ATCCTTGTGAGAAGAAATCGGCTGATGTGACTGTAGCTGCGTCTTTGCAAGGGAAGCCAT
TGATTTTGATTCCTGCAAAAAAGAAAAACAAGAAAGAGATTAGAAATTGAATCTTTTTCT
AGGAAAAGAGATGAAAGTAAAGGTTTTTGCAAGTACCAGAGGGAAGATCAGCGACGCAGA
GATCTTGAAGCATGTCAGGATCTGCGGAGGAGGAGATGAAGAAGGAGACGGTGGTGAGGA
GGAGGAGGAGAGTAAGAGTTGGTGAAGCCATTGGGATTGTGGTAGTGGTGGTGGTGGGAC
TCTCTCTAAACAAGTAGTTGTTGAGAGAGAAGAGAATTAATGAATTATGGTTTATAAAGG
GAGGAGAATTGAAGAATTGAAGAGAGGGTGACTTTTCTCGTTGAGGGAAAAAGCAAGCAA
CATGCTGTTTTTCTTTCTTGCACACATCTGTCTAAGTGAAATGTCAAAGGTCAACGAATT
ACTAAAACGGATGGATCAGAGCT

Last Updated on 06.12.2018 16:11