Sequences from confirmation amplicon


Confirmation sequences for line 436E12 and hit At2g39760 are shown.
Line ID 436E12
Locus At2g39760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16584085
Primers used for PCR BE82 - 8474, show primer details
   
Sequence Name 11-K029122-022-436-E12C-8409
GenBank Accession LN490979 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K029122-022-436-E12C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAAACGCTTCAATATATTCAT
TTATATGAGGTTTCCCGGACTGGAAATCGTTTCCAATTGGACATGAAGCGTTTGAAATTT
GTGATGATAAGTCATGATAGAGAAAACACCAACTTCCAGAAGGAAACCTCACTTTCAAGG
AAATGAGTCAATATATGAAATCAACAGATGATGCAAGAGTTCAAAAAAAGATTCACGAAA
CTTCAAAAGCAACATGCACACACCTTAAACCTCTCAAGGGATTCAAAAGCCATACTTTAA
AGAAAGAGAAAGAACAATATGAACCATACAATTAAAAAAACTTGTGCTTTACACAATTTT
AGCCGTAAGCAAACAAAAGCACAAACACATTGATAAAGTTCCTTAATTGCTTCTGACAGA
GAAGCACTAGCAGCTCATTCCACCACAAACATTTCTGCAAAATAAACTATCAACATGCAA
TTCAAGCAGAAGATAAACAACATTACTAAAACCTAAATGCAACAAGCCACAGTAACCTAA
CAAATGCGATTATATCATACTACAACAGCAAAATGAAATGGAAACGAAGCAATTACCACA
TGCTTCCTTTATACTTAAGTGTATAAGG
   
Sequence Name 12-K029122-022-436-E12C-BE82
GenBank Accession LN491439 [GenBank]
Sequencing primer gene specific primer, BE82: 5'-GCTTGAAGGTGGTCCTTATACACT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K029122-022-436-E12C-BE82
TAAGTATAAAGGAAGCATGTGGTAATTGCTTCGTTTCCATTTCATTTTGCTGTTGTAGTA
TGATATAATCGCATTTGTTAGGTTACTGTGGCTTGTTGCATTTAGGTTTTAGTAATGTTG
TTTATCTTCTGCTTGAATTGCATGTTGATAGTTTATTTTGCAGAAATGTTTGTGGTGGAA
TGAGCTGCTAGTGCTTCTCTGTCAGAAGCAATTAAGGAACTTTATCAATGTGTTTGTGCT
TTTGTTTGCTTACGGCTAAAATTGTGTAAAGCACAAGTTTTTTTAATTGTATGGTTCATA
TTGTTCTTTCTCTTTCTTTAAAGTATGGCTTTTGAATCCCTTGAGAGGTTTAAGGTGTGT
GCATGTTGCTTTTGAAGTTTCGTGAATCTTTTTTTGAACTCTTGCATCATCTGTTGATTT
CATATATTGACTCATTTCCTTGAAAGTGAGGTTTCCTTCTGGAAGTTGGTGTTTTCTCTA
TCATGACTTATCATCACAAATTTCAAACGCTTCATGTCCAATTGGAAACGATTTCCAGTC
CGGGAAACCTCATATAAATGAATATATTGAAGCGTTTATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGA

Last Updated on 06.12.2018 16:11