Sequences from confirmation amplicon


Confirmation sequences for line 437A12 and hit At1g10950 are shown.
Line ID 437A12
Locus At1g10950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3659214
Primers used for PCR NU15 - 8474, show primer details
   
Sequence Name 06-K103925-0022-437-A12C-NU15
GenBank Accession LN488783 [GenBank]
Sequencing primer gene specific primer, NU15: 5'-AACACAACTCAGTGTTGATCCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K103925-0022-437-A12C-NU15
AGAAATCACGAGAATCTCAAACCAACATCAAAATTTCTTATTAAAATCGAGATTGTGAAA
AGCCTAAATCACAATGAATAGACAAAATACGCCTAGACTTGCAACGAAAAGATACGCCTA
AATCACAATGAATAGAAGCTCGAGGATCCTAGCAATCCGTAAACGAACAGATCCATGAAA
AAAAATTCAATCGCAAACACCATGCAAATCACGGATCTACGGACACAGTGCTCGATTTCG
AGCTGCATTAAAAGAAAAGAAAAAACGACTGGAGATCCGAGTCTTACCTTGTGATCTGAA
TCGGATGCGAACGTAGGAGTGAGAAGGGAAACGAGAAGAAGGAACACGAGGACGGCGGCG
GAGGAGGAAGACGGCATCGTGAAAACCGGCGGACGATCAGATCTGAGGAAGGACGGAGCG
AGAGATACCAATCGAGAGAGAGCGAGATCCTCAGCTCAGTGTTTTCACTTTGTAGAGATT
TTGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TGATTACCCATTTTTTT
   
Sequence Name 22-K103926-0022-437-A12C-8409
GenBank Accession LN496085 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K103926-0022-437-A12C-8409
CAGTGAAACACTGAGCTGAGGATCTCGCTCTCTCTCGATTGGTATCTCTCGCTCCGTCCT
TCCTCAGATCTGATCGTCCGCCGGTTTTCACGATGCCGTCTTCCTCCTCCGCCGCCGTCC
TCGTGTTCCTTCTTCTCGTTTCCCTTCTCACTCCTACGTTCGCATCCGATTCAGATCACA
AGGTAAGACTCGGATCTCCAGTCGTTTTTTCTTTTCTTTTAATGCAGCTCGAAATCGAGC
ACTGTGTCCGTAGATCCGTGATTTGCATGG

Last Updated on 06.12.2018 16:11