Sequences from confirmation amplicon


Confirmation sequences for line 437F11 and hit At1g06310 are shown.
Line ID 437F11
Locus At1g06310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1927623
Primers used for PCR 13tq - 8474, show primer details
   
Sequence Name 82-K116920-0022-437-F11C-13tq
GenBank Accession LN514232 [GenBank]
Sequencing primer gene specific primer, 13tq: 5'-GTATGAAGCGGATCTTTTACTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K116920-0022-437-F11C-13tq
CGGGATTTATGATTACTCCCTCTCCGCCAAACTCGGTGTTCACTTCTTGTTGTGGTAATG
TTTCAAAAATCACATCTTTATTTTCTCATTTTTAGAGATAAGTAGAGAGATGTTGTTCAA
AATGAGGATTTGGAAAGTTCAGGGGTAATGCTGTTAAGTTCTTTGGGACAAAGCGTCACC
ATGAAAAGTGGCTGAAAGACACTGAAGATTATGTTGTCAAGGGCTGCTTTGCAATGACTG
AGCTAGGCCATGGAACTAATGTAGGCATATTGCAGTGTCATTTTGCATTTATCCAGACTA
TATATGTATTGGTGATCTTTGATTTGGTGGCTTTCAGGTACGGGGAATTGAAACAGTGAC
TACTTATGACCCAACAACTGAAGAGTTTGTGATAAATACTCCTTGTGAATCTGCTCAGAA
GTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATT
   
Sequence Name 38-K116919-0022-437-F11C-8409
GenBank Accession LN503124 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K116919-0022-437-F11C-8409
ACAAGGAGTATTTATCACAAACTCCTTCAGTTGTTGGGTCATAAGTAGTCACCGTTTCAA
TTCCCCGTACCTGAAAGCCACCAAATCAAAGATCACCAATACATATATAGTCTGGATAAA
TGCAAAATGACACTGCAATATGCCTACATTAGTTCCATGGCCTAGCTCAGTCATTGCAAA
GCAGCCCTTGACAACATAATCTTCAGTGTCTTTCAGCCACTTTTCATGGTGACGCTTTGT
CCCAAAGAACTTAACAGCATTACCCCTGAACTTTCCAAATCCTCATTTTGAACAACATCT
CTCTACTTATCTCTAAAAATGAGAAAATAAAGATGTGATTTTTGAAACATTACCACAACA
AGAAGTGAACACCGAGTTTGGCGGAGAGGGAGTAATCATAAATCCCGCAAACTTCGTAGA
GAGCAAACTTCTTGAGCTCAGCTTCAGGACCTGTCTCCGTTAGCCATCCTTGAAAGACCC
CTTTTTCGAGCAAGTAAAA

Last Updated on 06.12.2018 16:11