Sequences from confirmation amplicon


Confirmation sequences for line 437G06 and hit At3g23540 are shown.
Line ID 437G06
Locus At3g23540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8444821
Primers used for PCR 12lc - 8474, show primer details
   
Sequence Name 52-K113934-0022-437-G06C-12lc
GenBank Accession LN507884 [GenBank]
Sequencing primer gene specific primer, 12lc: 5'-CAATCAAGGATATGTTTCCATCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K113934-0022-437-G06C-12lc
CCTTCTGCAGCACTCACATCAGGTTAACATTAGTAAGAAGAAAAACCACTCAATAACTCA
GAATTATGTAAACAGTGACTTTATGGGTGAAGAATAATACACAGTTAACATGGTCAAAGA
CCAATACTAAAACCCAACATGAATTCCATGATTTCTCCACTTGTCAGTCTAAACATGAAC
TAGAGTTTCTATAGCCTTCAAATCAAATGAAATATGTCTTCATCAAGATGACAATAGGGT
ACTTCTATAGACAATAGCACAATCATATTGGTTTACACGGGTTTTAAATAAATAAAAATG
GAATAAATTTGAACAAACATGTTTACAAAGTAACAATATATTTTGAAGAGTAGAAGAGAT
AAACAAGAATGATTAAAGGAGCACATACTTCGTTCCAGCCTAAGGTGACATGTTCCCCGC
CTGAGAGACCCGATCCCGAAAAGTCAAGGGTAAAAACTGTGATGTTCGAAGGCAGCAACA
CAATTGCGGCTTCACTACCATCCGATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 17-K113933-0022-437-G06C-8409
GenBank Accession LN493933 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K113933-0022-437-G06C-8409
CGCATTGTGTTGCTGCCTTCGAACATCACAGTTTTTACCCTTGACTTTTCGGGATCGGGT
CTCTCAGGCGGGGAACATGTCACCTTAGGCTGGAACGAAGTATGTGCTCCTTTAATCATT
CTTGTTTATCTCTTCTACTCTTCAAAATATATTGTTACTTTGTAAACATGTTTGTTCAAA
TTTATTCCATTTTTATTTATTTAAAACCCGTGTAAACCAATATGATTGTGCTATTGTCTA
TAGAAGTACCCTATTGTCATCTTGATGAAGACATATTTCATTTGATTTGAAGGCTATAGA
AACTCTAGTTCATGTTTAGACTGACAAGTGGAGAAATCATGGAATTCATGTTGGGTTTTA
GTATTGGTCTTTGACCATGTTAACTGTGTATTATTCTTCACCCATAAAGTCACTGTTTAC
ATAATTCTGAGTTATTGAGTGGTTTTTCTTCTTACTAATGTTAAACCTGATGTGAGTGCT
TGCAGAAGGATGATCTGAAGGCTGTGGTTGAGTTTTTGCGGCAGGATGGAAACATACCCN
TGATTG

Last Updated on 06.12.2018 16:11