Sequences from confirmation amplicon


Confirmation sequences for line 437H04 and hit At5g66900 are shown.
Line ID 437H04
Locus At5g66900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26716990
Primers used for PCR 04st - 8474, show primer details
   
Sequence Name 81-K116920-0022-437-H04C-04st
GenBank Accession LN514110 [GenBank]
Sequencing primer gene specific primer, 04st: 5'-GATAATTGGAATCAAAACTCGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K116920-0022-437-H04C-04st
GAATCCGCTCCCCGCCACACATCATCCAACACCAACAATATAGGACCATTTTCTTTAAGT
TCCTCCAGCAGTTTTCTTAAGCCAACTTCTGCTCGAGAATCATTCTCAAACGTAAGTGCA
TTGTAACCGTTGTGCTGGAGTAAATTCTGTACTATTACCCTAAAGTTAGGAGTATTTGAC
ACAACATTAAAGAAGATATGCTTGAACTTCCCTGCCAAAGGAAACTACATGAGAAACCAG
CTCAACTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGT
   
Sequence Name 37-K116919-0022-437-H04C-8409
GenBank Accession LN502755 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K116919-0022-437-H04C-8409
AAATACTCCTAACTTTAGGGTAATAGTACAGAATTTACTCCAGCACAACGGTTACAATGC
ACTTAACATTTGAGAATGATTCTCAAGCAGAAGTTGGCTTAAGAAAACTGCTGGAGGAAC
TTAAAGAAAATGGTCCTATATTGTTGGTGTTGGATGATGTGTGGCGGGGAGCGGATTCTT
TCCTTCAGAAATTTCAAATTAAGTTACCAAATTATAAGATTTTGGTGACTTCTCGGTTTG
ACTTTCCGAGTTTTATCCCCAATTAT

Last Updated on 06.12.2018 16:11