Sequences from confirmation amplicon


Confirmation sequences for line 438E01 and hit At4g33830 are shown.
Line ID 438E01
Locus At4g33830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16220350
Primers used for PCR 17wz - 8474, show primer details
   
Sequence Name 36-K118924-0022-438-E01C-17wz
GenBank Accession LN502419 [GenBank]
Sequencing primer gene specific primer, 17wz: 5'-AAATTTCCCAACAATTCTTCAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K118924-0022-438-E01C-17wz
CTTATTTTGTCATCTAAACATTAAATTGTTTGCTAAATAAATTATTATTTTAATGTCTAA
ACTTTTCATAATGCTTATATGTAAGTGGTTTGATTAGTTTCCAAAACATACGCATTTGAT
CAACCTCCCTGCGAGTTTGACAATTAATGTTGGGTCAATATTATCAAAAAAAGCTAGAGA
ACGTTCAATATTATTGATAATAAATCCATTAGTATATATTAAGGAAACCAACCCAAAGAT
GCTCACGAAAGTTACCTCTCACACAAGTATAATTCAAAACAGACTATATATCTGCCACAA
CTTTTCACTAAGAATCAGACTACTCATATACATAAAGTAGTTGTAAACTTAGCGCCTCAA
ACACGAAAGACAAAAGTTGATGGAACTATCAGCAATTAAATGATAATCAGTCTATCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTA
   
Sequence Name 12-K118923-0022-438-E01C-8409
GenBank Accession LN491811 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118923-0022-438-E01C-8409
ATCCTGATAGACTGATTATCATTTAATTGCTGATAGTTCCATCAACTTTTGTCTTTCGTG
TTTGAGGCGCTAAGTTTACAACTACTTTATGTATATGAGTAGTCTGATTCTTAGTGAAAA
GTTGTGGCAGATATATAGTCTGTTTTGAATTATACTTGTGTGAGAGGTAACTTTCGTGAG
CATCTTTGGGTTGGTTTCCTTAATATATACTAATGGATTTATTATCAATAATATTGAACG
TTCTCTAGCTTTTTTTGATAATATTGACCCAACATTAATTGTCAAACTCGCAGGGAGGTT
GATCAAATGCGTATGTTTTGGAAACTAATCAAACCACTTACATATAAGCATTATGAAAAG
TTTAGACATTAAAATAATAATTTATTTAGCAAACAATTTAATGTATTAGATGACAAAATA
AAGACTAACTGATTTGTGGTCATTGGTCAAGCATTGTTGAAGAATTGTTGGGAAATTT

Last Updated on 06.12.2018 16:11