Sequences from confirmation amplicon


Confirmation sequences for line 439H04 and hit At3g18890 are shown.
Line ID 439H04
Locus At3g18890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6511354
Primers used for PCR NA58 - 8474, show primer details
   
Sequence Name 40-K101564-0022-439-H04C-8409
GenBank Accession LN503655 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K101564-0022-439-H04C-8409
GCCATTTACATTGAATATATCCTGACGCCATTTAAAATAAAAAGTTTCAAGCTTTTTGAT
TACACTACTTCTGCATCTTTTGGTTTGACTTTGTTTGCTTTTCGTGAAATGGGACTTTGC
TCGAACGTGATTCGCTTCTTCTCTTAACACTTTATCTGTGTCTTGTTTTTGCTGATGGTT
TCTGCAATGTTGTCGTTTCCCAAGTCTTTAATTCTAGTTTCAAAGTTCTCATCTTTTCAA
CCTTTTGATCAGACACCAGTAGTCTCATTATAATTTTTGGCGCCTGAAGCGCTAGCTTTA
CTGCAACATTTCTGGTATTGATTACCTGGAATTCATAGATTTTTACTCATTTCCCTACTT
GTTATTGAACAAAGGTCCCATTAGAGCAAGCTCTGTAGTAACAGAAGCAAGCCCTACCAA
CTTAAATTCCAAAGAAGAAGACCTTGTCTTTGTAGCCGGTGCTACTGGTAAAGTTGGTTC
TAGAACTGTGAGGTCAGTTTCTTGCTTCTTTAAGTTTGAATGATAATTCACAATGCTGGT
TGGTTGAACTGTCGAAGTGTCCTATCAATGTCGCCTTTCANACAGAGAGCTACTGAAGCT
GGG
   
Sequence Name 40-K101577-0022-439-H04C-NA58
GenBank Accession LN503656 [GenBank]
Sequencing primer gene specific primer, NA58: 5'-CCCAGCTTCAGTAGCTCTCTGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K101577-0022-439-H04C-NA58
TTCACCACCAGCATTGTGAATTATCATTCAAACTTAAAGAAGCAAGAAACTGACCTCACA
GTTCTAGAACCAACTTTACCAGTAGCACCGGCTACAAAGACAAGGTCTTCTTCTTTGGAA
TTTAAGTTGGTAGGGCTTGCTTCTGTTACTACAGAGCTTGCTCTAATGGGACCTTTGTTC
AATAACAAGTAGGGAAATGAGTAAAAATCTATGAATTCCAGGTAATCAATACCAGAAATG
TTGCAGTAAAGCTAGCGCTTCAGGCGCCAAAAATTATAATGAGACTACTGGTGTCTGATC
AAAAGGTTGAAAAGATGAGAACTTTGAAACTAGAATTAAAGACTTGGGAAACGACAACAT
TGCAGAAACCATCAGCAAAAACAAGACACAGATNAAGTGTTAAGAGAAGAAGCGAATCAC
GTTCGAGCAAAGTCCCATTTCACGAAAAGCAAACAAAGTCAAACCAAAAGATGCAGAAGT
AGTGTAATCAAAAAGCTTGAAACTTTTTATTTTAAATGGCTGTCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATATAGATNTCCG

Last Updated on 06.12.2018 16:11