Sequences from confirmation amplicon


Confirmation sequences for line 442A02 and hit At4g34110 are shown.
Line ID 442A02
Locus At4g34110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16336848
Primers used for PCR CD72 - 8474, show primer details
   
Sequence Name 10-K104268-0022-442-A02C-CD72
GenBank Accession LN490634 [GenBank]
Sequencing primer gene specific primer, CD72: 5'-AGAAACCGTAATGCTTCTTTTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K104268-0022-442-A02C-CD72
CTCTCGGCCCCCAAATATCTCACTCGCCGTCGCAGGCTTCGCCGCCGGCAATTTCTTACC
CTATTTCATCTTCCCTCTCCTCTGATATACCCATAAAAACACAAAAAAAACGAAAAGATA
AAAACTTTGGCTTTAGTTTTGTTGATTTTGTCATTCGATTCGATTTCTTTTTCTTCTTTC
AAATTCTAGGGTTTTCGATTTGGTTTCAGTCTAATAACGAGAGGGGAGGGAAGAGTAACT
AAAACCCCAAAAAAAGTCAAAAAAGTTTCGAAAATCCAAAAAAAGATAATGGCGCAGGTT
CAACTTCAGGGTCAGACTCCGAATGGTTCGACCGCGGCGGTCACTTCTGCTCCGGCGACG
TCNGGTGGAGCAACGGCGACCCAGTTTGGTAACACGTCGCTTTATTTGGCCAAACAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCA
   
Sequence Name 26-K104269-0022-442-A02C-8409
GenBank Accession LN497904 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104269-0022-442-A02C-8409
TCATTGAATATATCCTGTTTGGCCAATAAAGCGACGTGTTACCAAACTGGGTCGCCGTTG
CTCCACCTGACGTCGCCGGAGCAGAAGTGACCGCCGCGGTCGAACCATTCGGAGTCTGAC
CCTGAAGTTGAACCTGCGCCATTATCTTTTTTTGGATTTTCGAAACTTTTTTGACTTTTT
TTGGGGTTTTAGTTACTCTTCCCTCCCCTCTCGTTATTAGACTGAAACCAAATCGAAAAC
CCTAGAATTTGAAAGAAGAAAAAGAAATCGAATCGAATGACAAAATCAACAAAACTAAAG
CCAAAGTTTTTATCTTTTCGTTTTTTTTGTGTTTTTATGGGTATATCAGAGGAGAGGGAA
GATGAAATAGGGTAAGAAATTGCCGGCGGCGAAGCCTGCGACGGCAAGTGAGATATTTGG
GGGCCGAGAGATTCTAGGCGAAACAAGCACAATTACGATNGATGAAAAAGAAGCATTACG
GTTT

Last Updated on 06.12.2018 16:11