Sequences from confirmation amplicon


Confirmation sequences for line 443B12 and hit At5g05990 are shown.
Line ID 443B12
Locus At5g05990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1807423
Primers used for PCR 12mh - 8474, show primer details
   
Sequence Name 52-K114180-0022-443-B12C-12mh
GenBank Accession LN507889 [GenBank]
Sequencing primer gene specific primer, 12mh: 5'-GGGAAATAAAAACCCTCACTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K114180-0022-443-B12C-12mh
TAACAAAAATGGCGACTCTTGTAGTCGCGCAGCTTCTCGGTTTGTGGGAAGTTATTGGAA
AAACCGTTCTTTCGTTTACTCATTGTTGGACCAGTCCGCTTCTGGCCGGAGGCGACATCT
GACGCCGTCGGTTGACCGGAATGTTCCATTTACCACATCGACCAAAAAGAGAGCTTCTCC
AACTGATCCGCTTCTTCGAATCATTGAAGAGGAGATTGGATACGCCGAGAAAGCTGATGA
TTATCATCGAGTAAATCCTTACCATTTTCATGTTTTAGTTTCTGCAAATTTTGTTTATTA
AGATTTATGTGTTTCGTGAAGCTCTGTTTCAATGGCAATGGTGTGTAATCTCTCTGTTTC
TTCTTTTGTTTGAATAAAGGTTGAAGAAACTCCAAGTGGCTTCCCTTTCGAAATGGAAGA
CAAACCAGGAGGGAAGATTGTGACATTGACTAGAGAATATGAAGGAGAGACTGTTAAAGT
TGAAGTACACATGTCCGGGAAATCTACATGGATCAACATGAGTATGATGGTCAAATGGAG
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTANGAGTCCGCACAATTA
TT
   
Sequence Name 16-K114177-0022-443-B12C-8409
GenBank Accession LN493517 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114177-0022-443-B12C-8409
TTCACTTTACAGTCTCTCCTTCTATTCTCTAGTCAATGTCACAATCTTCCCTCCTGGTTT
GTCTTCCATTTCGAAAGGGAAGCCACTTGGAGTTTCTTCAACCTTTATTCAAACAAAAGA
AGAAACAGAGAGATTACACACCATTGCCATTGAAACAGAGCTTCACGAAACACATAAATC
TTAATAAACAAAATTTGCAGAAACTAAAACATGAAAATGGTAAGGATTTACTCGATGATA
ATCATCAGCTTTCTCGGCGTATCCAATCTCCTCTTCAATGATTCGAAGAAGCGGATCAGT
TGGAGAAGCTCTCTTTTTGGTCGATGTGGTAAATGGAACATTCCGGTCAACCGACGGCGT
CAGATGTCGCCTCCGGCCAGAAGCGGACTGGTCCAACAATGAGTAAACGAAAGAACGGTT
TTTCCAATAACTTCCCACAAACCGAGAAGCTGCGCGACGTACAAGAGTCGCCATTTTTTT
TGTGTTGAAGCTCAAATTGGAAGAGTGAGGGTTTTTATTTCC

Last Updated on 06.12.2018 16:11