Sequences from confirmation amplicon


Confirmation sequences for line 443D10 and hit At2g33500 are shown.
Line ID 443D10
Locus At2g33500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14190225
Primers used for PCR 03pt - 8474, show primer details
   
Sequence Name 40-K115290-0022-443-D10C-03pt
GenBank Accession LN503812 [GenBank]
Sequencing primer gene specific primer, 03pt: 5'-GAATTCATGGCTGGATTTAGGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K115290-0022-443-D10C-03pt
TTTCTAATTGCCAAATAATGATCATCTGGAACGATTTACGAGTTAGTAAACTTCGTACAT
GTGAAATATTCTCCTTATCGATCATGTCGTTAACATCCTAACGTCCCATCCAAATAATGG
GCTTAGAATTGGGCTTAGCAGAAACATGGGCTAAAATCCTTGTGCGTGGATCTGAAAGAA
AGGCAGCCAATTAATGTGCCTAAATCCATACACTGTAATGCAGCCAATTATTTCTCAAGT
AATTAATTACCTATGTAAATGCAACTTTATTTCCGGTACAACAGTTAGATATTTACAATT
GATGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATAGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATA
   
Sequence Name 16-K115289-0022-443-D10C-8409
GenBank Accession LN493554 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K115289-0022-443-D10C-8409
TACATTGATATATCCTGTCATCAATTGTAAATATCTAACTGTTGTACCGGAAATAAAGTT
GCATTTACATAGGTAATTAATTACTTGAGAAATAATTGGCTGCATTACAGTGTATGGATT
TAGGCACATTAATTGGCTGCCTTTCTTTCAGATCCACGCACAAGGATTTTAGCCCATGTT
TCTGCTAAGCCCAATTCTAAGCCCATTATTTGGATGGGACGTTAGGATGTTAACGACATG
ATCGATAAGGAGAATATTTCACATGTACGAAGTTTACTAACTCGTAAATCGTTCCAGATG
ATCATTATTTGGCAATTTAGAAAACAAATAAGTCAATATATATATACTAGGAACATATCC
TAAATCCGCCCATGAATT

Last Updated on 06.12.2018 16:11