Sequences from confirmation amplicon


Confirmation sequences for line 443G06 and hit At5g10330 are shown.
Line ID 443G06
Locus At5g10330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3248295
Primers used for PCR 66vs - 8474, show primer details
   
Sequence Name 86-K117999-0022-443-G06C-66vs
GenBank Accession LN514694 [GenBank]
Sequencing primer gene specific primer, 66vs: 5'-ACCACTCTAAACAACTCGAAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K117999-0022-443-G06C-66vs
TCCGAGTTTCTCTTTGATACGTATCATCAGCGAGGACGATCTGTTGAAGATTCTAGAAAT
GCCAATACTTGTTGTTCTAGATGAAGCTTACATCGAATTCTCAGGAGTTGAATCACGCAT
GAAATGGGTGAAGAAGTATGAAAACCTTATCGTTCTCCGCACATTTAGCAAACGAGCTGG
TAAATTTTGGTTTATCACTCTATATAATCTAAACATTACAGATATAAAGTCTGCATCTAA
GAATTCTGATGATATTTCAGGTTTAGCCGGGCTTCGAGTTGGATATGGAGCGTTTCCGTT
GAGTATAATCGAATACCTGTGGAGAGCAAAGCAACCGTACAATGTCTCTGTTGCAGGGGA
AGTAGCTGCTTTAGCAGCACTCTCAAACGGGAAATACTTGGAAGATGTCCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTAT
T
   
Sequence Name 46-K117998-0022-443-G06C-8409
GenBank Accession LN506000 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K117998-0022-443-G06C-8409
ATTTACAATTGGACATCTTCCAAGTATTTCCCGTTTGAGAGTGCTGCTAAAGCAGCTACT
TCCCCTGCAACAGAGACATTGTACGGTTGCTTTGCTCTCCACAGGTATTCGATTATACTC
AACGGAAACGCTCCATATCCAACTCGAAGCCCGGCTAAACCTGAAATATCATCAGAATTC
TTAGATGCAGACTTTATATCTGTAATGTTTAGATTATATAGAGTGATAAACCAAAATTTA
CCAGCTCGTTTGCTAAATGTGCGGAGAACGATAAGGTTTTCATACTTCTTCACCCATTTC
ATGCGTGATTCAACTCCTGAGAATTCGATGTAAGCTTCATCTAGAACAACAAGTATTGGC
ATTTCTAGAATCTTCAACAGATCGTCCTCGCTGATGATACTGTATCAAAGAGAAACTCGG
ATTAATAATCATGACGGACTACTAAAAAGAATTTTTCCTTTCGAGTTGTTAAGAGTGG

Last Updated on 06.12.2018 16:11