Sequences from confirmation amplicon


Confirmation sequences for line 444C09 and hit At5g57630 are shown.
Line ID 444C09
Locus At5g57630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23342089
Primers used for PCR DB23 - 8474, show primer details
   
Sequence Name 09-K030219-022-444-C09C-8409
GenBank Accession LN490053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K030219-022-444-C09C-8409
GCCATTTACAATTGAATATTTGCTCTCCAGTGTATGTAACAAGAAACAGATGGGAATCCA
GTGAATCCAGGTGGGAACGTGTAATCTGCTCTGAGTATCTTCTTATACAAAACCGGAAGT
GTATGATCATCGAAAGGTGGATAACCAGCAAGCAATTCAAATAGAATCACTCCACAAGAC
CATACATCTACTGCTGCTCCTGAGTAGCCTTTGTTCATAATCAACTGTTTTCATTAAACA
AAATCAGCTTCTGCAGATTCAAAGATAGAGGTAAATGATTGTTTTTTGTGAATGTTAGTA
TTACCTCTGGTGCTATATAACATGGAGAGCCACAAGCTGTAGAGAGCATATCCCCCGACT
GTGAATTACATAAACCAAAAGTCTTATTTAGCATCCTTTGATTGTTACAAACCCAA
   
Sequence Name 10-K030219-022-444-C09C-DB23
GenBank Accession LN490527 [GenBank]
Sequencing primer gene specific primer, DB23: 5'-ATTTGGGTTTGTAACAATCAAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K030219-022-444-C09C-DB23
TTGGTATGTATTCACAGTCGGGGGATATGCTCTCTACAGCTTGTGGCTCTCCATGTTATA
TAGCACCAGAGGTAATACTAACATTCACAAAAAACAATCATTTACCTCTATCTTTGAATC
TGCAGAAGCTGATTTTGTTTAATGAAAACAGTTGATTATGAACAAAGGCTACTCAGGAGC
AGCAGTAGATGTATGGTCTTGTGGAGTGATTCTATTTGAATTGCTTGCTGGTTATCCACC
TTTCGATGATCATACACTTCCGGTTTTGTATAAGAAGATACTCAGAGCAGATTACACGTT
CCCACCTGGATTCACTGGATTCCCATCTGTTTCTTGTTACATACACTGGAGAGCAAATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATT

Last Updated on 06.12.2018 16:11