Sequences from confirmation amplicon


Confirmation sequences for line 444G09 and hit At1g24190 are shown.
Line ID 444G09
Locus At1g24190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8568132
Primers used for PCR AB31 - 8474, show primer details
   
Sequence Name 46-K103787-0022-444-G09C-AB31
GenBank Accession LN505846 [GenBank]
Sequencing primer gene specific primer, AB31: 5'-AAAGAGAGGAATAATGGCTCACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K103787-0022-444-G09C-AB31
GTCAGTAACCAATTTTTTTTTTTAAGTATGGAATATAGGTTTCCAGTCCACCATTAGAAG
TTGATTAACCAACCTGTCATCCTCACATTTGAAGAGGCTCTCTTCATACTGGTTCTTACG
CAGGTGGCTAAATGAATAATCTTCACTTCCTGATGTGACGGAAACCCAGTGATCATTAAA
AACCAGTTTACCGATCTCTGTCTTCTGGCTTGCAATAAAAATTGGGTACTGCAAAAAGTA
TAATGTCTTACATTAAACTAATGAAACAAAAGATGAACTCAAGAATAGTAAATCCACATA
TAAAAGGCTGCAGCCACATACTTTCTTTGGAAGTAACCGATAACTAGGAGTGCATTGTTC
ACAGTTCGAGAGATCTAGTTCACTGATTGGCTTTGCCCATTTCAAATTTGCAGCTGCTTT
TTCAAGCCTTTCCTTTTCATGATCTCTATCTCTAAGCCCGTCATCTCTCTAATGTTCCTG
GTCTCTATCATTGTCCAGTGACGGTTGAGGATATTTGCCTTCACTCCACAAGGATTCTGC
AGTATAGTTTAAAATGGATTTGTTTTCTTTTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTT
   
Sequence Name 62-K103788-0022-444-G09C-8409
GenBank Accession LN510743 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K103788-0022-444-G09C-8409
ATTTACATTGAATATATCCTGAAAAGAAACAAATCCATTCTAAACTATACTGCAGAATCC
TTGTGGAGTGAAGGCAAATATCCTCAACCGTCACTGGACAATGATAGAGACCAGGAACAT
AAGAGAGATGACGGGCTTAGAGATAGAGATCATGAAAAGGAAAGGCTTGAAAAAGCAGCT
GCAAATCTGAAATGGGCAAAGCCAATCAGTGAACTAGATCTCTCGAACTGTGAACAATGC
ACTCCTAGTTATCGGTTACTTCCAAAGAATGTATGTGGCTGCAGCCTTTTATATGTGGAT
CTACTATTCTTGAGTTCATCTTTTGTTTCATTAGTCTAATGTAAGACATTATACTTTTTG
CAGTACCCAATTTCTATTGCAAGCCAGAAGACAGAGATCGGTAAACTGGTTCTTAATGAT
CACTGGGTCTCCGTCACATCAGGAAGTGAAGATTATTCATTTAGCCACATGCGTAAGAAC
CAGTATGAAGAGAGCCTCTTCAAATGTGAGGATGACAGGTTGGTTAATCAACTTCTAATG
GTGGACTGGAAACCTATATTCCATACTTTAAAAAAAAAAATTGGTTACTGACCTGTTATT
GTGAGCCATTTTCTGTGTCAAATTGTGAGCCATTATTCCTCTCT
Primers used for PCR AB32 - 8474, show primer details
   
Sequence Name 39-K104195-0022-444-G09C-AB32
GenBank Accession LN503339 [GenBank]
Sequencing primer gene specific primer, AB32: 5'-CAGAACTGCAATCTTTGGTATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K104195-0022-444-G09C-AB32
TTGTTTGCATGATTCATGAACATTTGTGCTTGTATCAGGTAGGGAATTTAATCGGAGTAT
ATCCTGACCTCATGGATTCCTTTATAGAGTTTTTGGTGCAATGTGAAAAGAATGGTAACG
AGGCAAATCTATGTTTCTCTCTTCCTACATTCATTGATTTCAGAAAAAAGACAGATTTGT
AACCTTTTGAATCTTCTTGCAGAAGGACTGCTCTCTGGTATTCTGACTAAGAGTAAGTCT
ACTTATCTTTTGCAAGGGTACATGAATCTGATGCTGAGATATATTTGAAGTATTCACGAG
TAGTGAAACTACACAACTCCACTATGAAATTTGATAAATCCGGATATTTGTTTTCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGC
   
Sequence Name 55-K104196-0022-444-G09C-8409
GenBank Accession LN508799 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K104196-0022-444-G09C-8409
TCGGAATGAGCCTTTACATTGAATATATCCTGAAAACAAATATCCGGATTTATCAAATTT
CATAGTGGAGTTGTGTAGTTTCACTACTCGTGAATACTTCAAATATATCTCAGCATCAGA
TTCATGTACCCTTGCAAAAGATAAGTAGACTTACTCTTAGTCAGAATACCAGAGAGCAGT
CCTTCTGCAAGAAGATTCAAAAGGTTACAAATCTGTCTTTTTTCTGAAATCAATGAATGT
AGGAAGAGAGAAACATAGATTTGCCTCGTTACCATTCTTTTCACATTGCACCAAAAACTC
TATAAAGGAATCCATGAGGTCAGGATATACTCCGATTAAATTCCCTACCTGATACAAGCA
CAAATGTTCATGAATCATTGCAAACAAATGCCACAGTGAAAATTTAAGCATTTTATTTAT
TCATACCAAAGATGGCAGTTCTG

Last Updated on 06.12.2018 16:11