Sequences from confirmation amplicon


Confirmation sequences for line 444H07 and hit At3g13672 are shown.
Line ID 444H07
Locus At3g13672 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4474318
Primers used for PCR 15du - 8474, show primer details
   
Sequence Name 74-K110680-0022-444-H07C-15du
GenBank Accession LN513094 [GenBank]
Sequencing primer gene specific primer, 15du: 5'-ACCCAATTGGATACTTGCTATGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K110680-0022-444-H07C-15du
GAACCTTGACAATTTGTAATTTCATCCCAAGTCATGTTACTATATGTTTTTTTGTTTGTA
TTATTTTCTCTCCTACAATTAAGCTCTTTGACGTACGTAATCTCCGGAACCAACTCCTAT
ATCCACCATTTACTCCACGTTGTCTCCAATTATTGGACGTTGAAACTTGACACAACGTAA
ACGTATCTATGTGGTTGATTGTATGTACATATGTACAAACGTACACCTTTCTCCTCTTTC
ACTTCATCACTTGGCTTGTGAATTCATTAATTCCTGCGAAATGGAACCTCGAATCAATGA
CCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGACACGTTATA
   
Sequence Name 66-K110679-0022-444-H07C-8409
GenBank Accession LN511637 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K110679-0022-444-H07C-8409
GCCATTTACAATTGAAGGTCATTGATTCGAGGTTCCATTTCGCAGGAATTAATGAATTCA
CAAGCCAAGTGATGAAGTGAAAGAGGAGAAAGGTGTACGTTTGTACATATGTACATACAA
TCAACCACATAGATACGTTTACGTTGTGTCAAGTTTCAACGTCCAATAATTGGAGACAAC
GTGGAGTAAATGGTGGATATAGGAGTTGGTTCCGGAGATTACGTACGTCAAAGAGCTTAA
TTGTAGGAGAGAAAATAATACAAACAAAAAAACATATAGTAACATGACTTGGGATTGAAA
TTACAAATTAGTCAAGGTTCTTAGTTACACGACACTCTTGAGTTGGAGTTGTCATAGCAA
GTATCCAATTGGG

Last Updated on 06.12.2018 16:11