Sequences from confirmation amplicon


Confirmation sequences for line 446A08 and hit At3g61600 are shown.
Line ID 446A08
Locus At3g61600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22795806
Primers used for PCR NC56 - 8474, show primer details
   
Sequence Name 41-K101269-0022-446-A08C-8409
GenBank Accession LN504012 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K101269-0022-446-A08C-8409
CGGAATGAGCATTTACATTGAATATTCCAAATCGAATCCAAATCGCCGTCGGAGGAAGAA
CCATGACGAGTGAAGTCAGGATCCATTTGGGTTTTGGGATCGAACAGATCCGTATTCTCA
GTAGTACCTCTCATCACCTTCCTTCAATTAATTTCCTAAGCCGGAGAAACTGATCGGAGA
GCTTTTGATTTTGGAAAGAGAAAGCTGAACGTCTTTCTTTCAGGTGAGAGATAGACGAAA
GGCTCAACGAGACCAAAGTGATGACTGATTGTCTGTCTTCTTATCTTTCTATGAACCGGT
TTATGACTTGGTTCGGTTTGA
   
Sequence Name 41-K101275-0022-446-A08C-NC56
GenBank Accession LN504013 [GenBank]
Sequencing primer gene specific primer, NC56: 5'-ATCAAACCGAACCAAGTCATAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K101275-0022-446-A08C-NC56
ACATCAGTCATCACTTTGGTCTCGTTGAGCCTTTCGTCTATCTCTCACCTGAAAGAAAGA
CGTTCAGCTTTCTCTTTCCAAATCAAAAGCTCTCCGATCAGTTTCTCCGGCTTAGGAAAT
TAATTGAAGGAAGGTGATGAGAGGTACTACTGAGAATACGGATCTGTTCGATCCCAAAAC
CCAAATGGATCCTGACTTCACTCGTCATGGTTCTTCCTCCGACGGCGATTTTGGATTCGA
TTTTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11