Sequences from confirmation amplicon


Confirmation sequences for line 446D05 and hit At3g01540 are shown.
Line ID 446D05
Locus At3g01540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:214013
Primers used for PCR 40ve - 8474, show primer details
   
Sequence Name 54-K117914-0022-446-D05C-40ve
GenBank Accession LN508615 [GenBank]
Sequencing primer gene specific primer, 40ve: 5'-CCAAGAAGAAGCTGTTAAATTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K117914-0022-446-D05C-40ve
TCTCTGACTTGTAAGTTTCAACATGCTACTTGTGAAGTATATGTCTAACGTATGCCAAAT
GTGTGGTCTTATTATCTCTGTTTCAGTGTTTGTATGGTGGTGCACCAAAGGGCCCTCAGT
TGAGGGATTTAGAAAGAGGAGCAGATATCGTGGTTGCAACTCCTGGGAGATTGAATGATA
TCCTTGAAATGAGGAGGATTAGTCTGCGTCAGATATCTTATCTTGTGCTAGATGAGGCAG
ATAGAATGTTGGACATGGGTTTTGAACCTCAGATAAGGAAGATTGTGAAAGAAATTCCCA
CTAAGCGTCAAACCCTTATGTACACAGCTACATGGCCAAAGGGAGTTAGGAAAATTGCAG
CTGACTTGCTTGTTAACCCTGCTCAAGTCAACATTGGCAATGTTGACGAGCTTGTGGCTA
ACAAGTCAATCACACAGGTATTGCTTGAACTGATTCTATTGGTTCTGCTGACTGTTTCAG
GAGAATCTGATTGTTGTGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCG
   
Sequence Name 14-K117913-0022-446-D05C-8409
GenBank Accession LN492700 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117913-0022-446-D05C-8409
CACAATCAGATTCTCCTGAAACANTCAGCAGAACCAATAGAATCAGTTCAAGCAATACCT
GTGTGATTGACTTGTTAGCCACAAGCTCGTCAACATTGCCAATGTTGACTTGAGCAGGGT
TAACAAGCAAGTCAGCTGCAATTTTCCTAACTCCCTTTGGCCATGTAGCTGTGTACATAA
GGGTTTGACGCTTAGTGGGAATTTCTTTCACAATCTTCCTTATCTGAGGTTCAAAACCCA
TGTCCAACATTCTATCTGCCTCATCTAGCACAAGATAAGATATCTGACGCAGACTAATCC
TCCTCATTTCAAGGATATCATTCAATCTCCCAGGAGTTGCAACCACGATATCTGCTCCTC
TTTCTAAATCCCTCAACTGAGGGCCCTTTGGTGCACCACCATACAAACACTGAAACAGAG
ATAATAAGACCACACATTTGGCATACGTTAGACATATACTTCACAAGTAGCATGTTGAAA
CTTTACAAGTTCAGAGACTTCTACATACCGTACACGAAATTCTTGATGACCTCCCAAATT
TAACAGCTTCTTCATGGAAAAAAAA

Last Updated on 06.12.2018 16:11