Sequences from confirmation amplicon


Confirmation sequences for line 446E04 and hit At2g26730 are shown.
Line ID 446E04
Locus At2g26730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11391165
Primers used for PCR EQ70 - 8474, show primer details
   
Sequence Name 65-K103357-0022-446-E04C-EQ70
GenBank Accession LN511378 [GenBank]
Sequencing primer gene specific primer, EQ70: 5'-GGTTATCATGCTCCTGAAGTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K103357-0022-446-E04C-EQ70
TTTCGGGGTGTTGCTTCTTGAACTGTTAACAGGAAAATCACCAAACCAGGCATCACTTGG
GGAAGAAGGTATTGATCTACCTCGGTGGGTACTCTCCGTGGTTCGAGAAGAATGGACTGC
TGAAGTATTTGACGTTGAGCTGATGAGGTACCACAACATAGAGGAAGAGATGGTTCAGCT
TCTTCAAATCGCAATGGCTTGTGTATCAACGGTTCCTGATCAACGACCAGTAATGCAAGA
AGTACTAAGAATGATCGAAGATGTTGACCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 68-K103358-0022-446-E04C-8409
GenBank Accession LN511992 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K103358-0022-446-E04C-8409
CCATTTACAATTGAATATATCCTGGGGTCAACATCTTCGAAGTCATTCTTAGTACTTCTT
GCATTACTGGGGTCGTTGATCAGGGAACCGNNTTGATACACANNAGCCATTGCGNNNATT
TGAAGAAGCTGAACCNATCTCNTNNTCCTCTATGTTGTGGGTACNNCTCATCAGCTCAAC
NGTCAANNATACTTCAGCAGTCNNCATTCTTCTCGAACCACNGGAGAAGTACNNCCACCG
AGGTAGATCAATANNCCTTCTTCCCNNCAAGTGATGCCTGGGGTTTGGTGATTTTCCTGG
TTAACANGTTCAAGAAAGCAACACNNCCCGAAACTGGTACACNATCCGAATTTAAAANNG
TTACTTTCCGTGGTCTCNGAGAACTNTCAGGAGCANNTGATAAC

Last Updated on 06.12.2018 16:11