Sequences from confirmation amplicon


Confirmation sequences for line 446E11 and hit At2g23400 are shown.
Line ID 446E11
Locus At2g23400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9964378
Primers used for PCR 79pv - 8474, show primer details
   
Sequence Name 44-K115481-0022-446-E11C-79pv
GenBank Accession LN505288 [GenBank]
Sequencing primer gene specific primer, 79pv: 5'-TACAATGAAACGTGAAACAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K115481-0022-446-E11C-79pv
ATAAGAGGATGAAGGTGGATCCACGCGACAACCAAATCGTCTTTCCCTGCCTTGATACGA
AGCGAGGGCCTCTAGAAGCTTATCCTTATCGAAATCAGGCCACAATACCGGCGAGTAGAA
AAGCTCGGTATAAGCAAGTTGCCACAAGAAGAAGTTACTAATCCTTTGTTCTCCACTTGT
CCTAATCATTAAATCAGGACTTGGGAAGTCACTACAATTCGTTAGAAGCTCTCTCTCGAT
CAATGCCTCGTCTACATCTTCCTCTCGGATCAACCCTTTTTCTGATTTCTTCACAAGACT
CTTGCAAGCATGCATGATATCAAATTTCCCGCTGTAATCTACTGCCATGATGAGATGCTT
ATTCTTGTAGCCTTGGTAGCTTCCTCTATCTCATGGATTTCTTTGAGGAGAGATTCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATG
   
Sequence Name 16-K115480-0022-446-E11C-8409
GenBank Accession LN493560 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K115480-0022-446-E11C-8409
ATCCTGAATCTCTCCTCAAGAAATCCATGAGATAGAGGAAGCTACCAAGGCTACAAGAAT
AAGCATCTCATCATGGCAGTAGATTACAGCGGGAAATTTGATATCATGCATGCTTGCAAG
AGTCTTGTGAAGAAATCAGAAAAAGGGTTGATCCGAGAGGAAGATGTAGACGAGGCATTG
ATCGAGAGAGAGCTTCTAACGAATTGTAGTGACTTCCCAAGTCCTGATTTAATGATTAGG
ACAAGTGGAGAACAAAGGATTAGTAACTTCTTCTTGTGGCAACTTGCTTATACCGAGCTT
TTCTACTCGCCGGTATTGTGGCCTGATTTCGATAAGGATAAGCTTCTAGAGGCCCTCGCT
TCGTATCAGGGCAGGGAAAGACGATTTGGTTGTCGCGTGTGATCCACCTTTCATCCTCTT
TATTCATCTATAATTTCTATCAAACTACTTGCTCTTTGTTTC

Last Updated on 06.12.2018 16:11