Sequences from confirmation amplicon


Confirmation sequences for line 447C01 and hit At4g27240 are shown.
Line ID 447C01
Locus At4g27240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13640697
Primers used for PCR 15wu - 8474, show primer details
   
Sequence Name 40-K118761-0022-447-C01C-15wu
GenBank Accession LN503877 [GenBank]
Sequencing primer gene specific primer, 15wu: 5'-ACGATACAGAGAGAAAACACCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K118761-0022-447-C01C-15wu
GGTTTTCACTTAAGAAATCTCTCCCCTGCAAATCTGATGTCTCCGATGTCCACATTCCGA
GATCCAAAAAAGAGCTAGCTCCCATATCCACTAAGAGAACAACAACCTCCTCCGGCGGCG
GGGTCGGCGGCAGGTCCGGTTGTTCAAGATCCATAGCCAATCTTAAAGACGTAATCCATG
GAAACCAACGGCATTTAGAGAAGCCGCTTTGCTCTAGCCCTCGTTCTATAGGAAGCAGCG
AGTTTCTCAATCCCATTACTCACGATGTAATCTTTAGCAACTCCACCTGCGAGCTCAAGA
TCACCGCCGCCGGAGCAACAGAATTCGTCGGGAATCTTAGACCAGGGACGCCGGTCAATT
ACTCTTCTTCACGCCGTAGCCAAACTTCGAGAAAGGCTTCTTCTTTAGATAGAGAAGGAT
TAGGGTTTCACCAGAGCATGATAGAGAAGCCGCCATTAACGACAAATTGGTAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCA
   
Sequence Name 15-K118760-0022-447-C01C-8409
GenBank Accession LN493194 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118760-0022-447-C01C-8409
GCCATTTACAATTGAATTATCCTACCAATTTGTCGTTAATGGCGGCTTCTCTATCATGCT
CTGGTGAAACCCTAATCCTTCTCTATCTAAAGAAGAAGCCTTTCTCGAAGTTTGGCTACG
GCGTGAAGAAGAGTAATTGACCGGCGTCCCTGGTCTAAGATTCCCGACGAATTCTGTTGC
TCCGGCGGCGGTGATCTTGAGCTCGCAGGTGGAGTTGCTAAAGATTACATCGTGAGTAAT
GGGATTGAGAAACTCGCTGCTTCCTATAGAACGAGGGCTAGAGCAAAGCGGCTTCTCTAA
ATGCCGTTGGTTTCCATGGATTACGTCTTTAAGATTGGCTATGGATCTTGAACAACCGGA
CCTGCCGCCGACCCCGCCGCCGGAGGAGGTTGTTGTTCTCTTAGTGGATATGGGAGCTAG
CTCTTTTTTGGATCTCGGAATGTGGACATCGGAGACATCAGATTTGCAGGGGAGAGATTT
CTTAAGTGAAAACCAAACACTGGGTAGCTTTTTTTTCTTTTGAGTATCGGTGTTTTCTCC
ATGTATCG

Last Updated on 06.12.2018 16:11