Sequences from confirmation amplicon


Confirmation sequences for line 447H07 and hit At4g00660 are shown.
Line ID 447H07
Locus At4g00660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:276166
Primers used for PCR SR30 - 8474, show primer details
   
Sequence Name 47-K103544-0022-447-H07C-SR30
GenBank Accession LN506175 [GenBank]
Sequencing primer gene specific primer, SR30: 5'-AAAGCTGGCGTCGTAAATAGATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K103544-0022-447-H07C-SR30
TATAAAACTTAGTGAATGTAAATCGTAATGAATCCTTTGAGCCTCTAAACCCATTGCTTC
TATCAGTAGCCTAATATGTTTCTTAAAGGTTTACTGCTTCCTTTCTAACTTAGTTTACCG
TCAATACCTTTCTTGTACGCAGCTGTTATAATTGTTCCGACTCGAGAGTTAGCCCTTCAG
ACATCACAGGTCTGTAAAGAGCTGGGTAAGCATTTGAAGATTCAAGTCATGGTGACCACT
GGTGGCACCAGTCTGAAAGACGATATCATGCGTTTGTATCAGCCTGTTCATTTACTGGTA
GGAACTCCTGGGAGAATTCTGGATCTTACCAAGAAAGGTGTTTGTGTTTTAAAAGAAAAA
GAAAAAGAAAGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACAATTATT
   
Sequence Name 63-K103545-0022-447-H07C-8409
GenBank Accession LN510967 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K103545-0022-447-H07C-8409
TCTTTCTTTTTCTTTTTCTTTAAAACACAAACACCTTTCTTGGTAAGATCCAGAATTCTC
CCAGGAGTTCCTACCAGTAAATGAACAGGCTGATACAAACGCATGATATCGTCTTTCAGA
CTGGTGCCACCAGTGGTCACCATGACTTGAATCTTCAAATGCTTACCCAGCTCTTTACAG
ACCTGTGATGTCTGAAGGGCTAACTCTCGAGTCGGAACAATTATAACAGCTGCGTACAAG
AAAGGTATTGACGGTAAACTAAGTTAGAAAGGAAGCAGTAAACCTTTAAGAAACATATTA
GGCTACTGATAGAAGCAATGGGTTTAGAGGCTCAAAGGATTCATTACGATTTACATTCAC
TAAGTTTTATACAGTAACTAGTGTTTATTGTGGATCAATAATCTATTTACGACGC

Last Updated on 06.12.2018 16:11