Sequences from confirmation amplicon


Confirmation sequences for line 448E01 and hit At1g23900 are shown.
Line ID 448E01
Locus At1g23900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8442985
Primers used for PCR 35rb - 8474, show primer details
   
Sequence Name 34-K115914-0022-448-E01C-35rb
GenBank Accession LN501547 [GenBank]
Sequencing primer gene specific primer, 35rb: 5'-CATACAGGAGCATCGACGTAATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K115914-0022-448-E01C-35rb
CATTTTTTCTCCTCGCAATAAAGTTTGATATCCTGTGGTATTCACTTATAAATAGATTAC
TCTGCTGAAATTGAGGCAGACAATCAGGTTGTTATATTGTTTTATCAGACCGTCAGTTTT
GACAAACCCTTGTCTTTGTTTTGTCTCTGCAGAAATGCACAGAGGGACTGATAAAAACTT
TGAGGGATATTACTAATAGCGCATATCAGCCTGAATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 08-K115913-0022-448-E01C-8409
GenBank Accession LN489876 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K115913-0022-448-E01C-8409
ATATTCAGGCTGATATGCGCNTATTAGTAATATCCCTCAAAGTTTTTATCAGTCCCTCTG
TGCATTTCTGCAGAGACAAAACAAAGACAAGGGTTTGTCAAAACTGACGGTCTGATAAAA
CAATATAACAACCTGATTGTCTGCCTCATTTCAGCAGAGTAATCTATTTATAAGTGAATA
CCACAGATATCAAACTTTATTGCAAGAGAAAAATAGTACACAAAAGCATCATACAGGTAA
TATAGTATCATATGTACGCATATTCAATTAAATTACGTCGATCGCTTCCTGTATG

Last Updated on 06.12.2018 16:11