Sequences from confirmation amplicon


Confirmation sequences for line 448E10 and hit At1g71830 are shown.
Line ID 448E10
Locus At1g71830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27020812
Primers used for PCR BD50 - 8474, show primer details
   
Sequence Name 48-K028245-022-448-E10C-8409
GenBank Accession LN506403 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K028245-022-448-E10C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTTTGAAACTGGA
GCTCTCCACCTGGAGTTCGCTCTTCCTTCAGTCTCTTGACAGCAACAAGAGTTCCGTCTG
CCAAGCGTCCCTTGTAGACTTTCCCAAACCCACCTCTGCCCAAAATGTTCTTGTTACTAA
ACCCATCACTCGCCACTTGTAGCTCCCGCAAAGAAAACCTCTTGAGCTGTCCCAGATGAA
CTTCTGGATCTTCTTCGGCTGCAAAGAGATTTTGCAATCGAGATTTTACTCAAAAGAGTT
TGTAACTTAAAAAACAATGACTTATGTATTGATTTTCATTAACAGTTGTTCCAATACTTT
TCTGCAAAACAAAATTG
   
Sequence Name 49-K028245-022-448-E10C-BD50
GenBank Accession LN506718 [GenBank]
Sequencing primer gene specific primer, BD50: 5'-AGCAATTTTGTTTTGCAGAAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K028245-022-448-E10C-BD50
TTGGAACAACTGTTAATGAAAATCAATACATAAGTCATTGTTTTTTAAGTTACAAACTCT
TTTGAGTAAAATCTCGATTGCAAAATCTCTTTGCAGCCGAAGAAGATCCAGAAGTTCATC
TGGGACAGCTCAAGAGGTTTTCTTTGCGGGAGCTACAAGTGGCGAGTGATGGGTTTAGTA
ACAAGAACATTTTGGGCAGAGGTGGGTTTGGGAAAGTCTACAAGGGACGCTTGGCAGACG
GAACTCTTGTTGCTGTCAAGAGACTGAAGGAAGAGCGAACTCCAGGTGGAGAGCTCCAGT
TTCAAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11