Sequences from confirmation amplicon


Confirmation sequences for line 449G08 and hit At4g00230 are shown.
Line ID 449G08
Locus At4g00230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:95254
Primers used for PCR 34jx - 8474, show primer details
   
Sequence Name 76-K113379-0022-449-G08C-34jx
GenBank Accession LN513431 [GenBank]
Sequencing primer gene specific primer, 34jx: 5'-CCGAGATCTATTTTGCTCTTGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K113379-0022-449-G08C-34jx
CCGGCTCATGGTTCGTTACGTTCCTGAGCTAGGCCCGTCGTTACCGGCGGACGCCACCGT
GAGGATTCCTTTCCTCATCGCGTGAAACGACCCGACGGATATCGAATCGGAAGAGTAATC
GGCGATTGGACCGCCGATAGAGATGGAGATAATTTCTACGCCGTCGTGAATCGCTGCTTC
GAATCCGGCGAGTATGTCCATGTCAGCACAGCCGGATCTCGCCCAACAAACCTTGTACAT
CGCTAACCTCGCCGACGGAACCGCGCCGCGGGCGGTGCCGTTTGCTATGCCATAGAGACT
CGCGTTTGCGACTAAAACGCCGGCTACGGTGGATGACGTGTGCGTCCCATGTCCGTCGAT
ATCGATCGGTGATCGGACTTCAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 38-K113377-0022-449-G08C-8409
GenBank Accession LN503050 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K113377-0022-449-G08C-8409
TTGAATTGAAGTCCGATCACCGATCGATATCGACGGACATGGGACGCACACGTCATCCAC
CGTAGCCGGCGTTTTAGTCGCAAACGCGAGTCTCTATGGCATAGCAAACGGCACCGCCCG
CGGCGCGGTTCCGTCGGCGAGGTTAGCGATGTACAAGGTTTGTTGGGCGAGATCCGGCTG
TGCTGACATGGACATACTCGCCGGATTCGAAGCAGCGATTCACGACGGCGTAGAAATTAT
CTCCATCTCTATCGGCGGTCCAATCGCCGATTACTCTTCCGATTCGATATCCGTCGGGTC
GTTTCACGCGATGAGGAAAGGAATCCTCACGGTGGCGTCCGCCGGTAACGACGGGCCTAG
CTCAGGAACTGTAACGAACCATGAGCCGTGGATATTGACGGTTGCTGCAAGTGGAATCGA
TCGGACGTTCAAGAGCAAAAAGGATCTCGG

Last Updated on 06.12.2018 16:11