Sequences from confirmation amplicon


Confirmation sequences for line 450D01 and hit At1g60800 are shown.
Line ID 450D01
Locus At1g60800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22385121
Primers used for PCR JC24 - 8474, show primer details
   
Sequence Name 15-K102588-0022-450-D01C-8409
GenBank Accession LN492922 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K102588-0022-450-D01C-8409
CCATTTTCAGTGTCAGTTTTGCTCTGCACAAAATGATGTGAAGCCCATTTTATTTCAAGA
TACTAACCATTAACGTCAAAAAATATTTGCTTGTTACGGCGATATCTCCACCAAAGAAAC
ATTCCGCTTGTAAAGAAAACAAAAAATGCTGCACTGAAGCTTGCGGCAAATGCAAGAGCA
ACGTGATGGCCATTGGTACGAGTTCCTGATTCATCTAAAACAGAAAGAAGAACCAAAGAT
GGAAGTGATGAGATACATTGACGCAGAAGAAGAATAGGGAGCTATGAGAAGCAGATGCCT
AATACCTGGACCATCTTGTGGAAGCGTGAGAGGCTCGGGAACAGCAGAACAGTTTGAAAC
AGCTTTTGGGCCACAGATTAACGCATTACCAATTACCCTTCAGTTTAAGAAGCCCAACGC
TGAATCTTAGTTTCAGCTTCAACAGAAGAATAAGTT
   
Sequence Name 15-K102589-0022-450-D01C-JC24
GenBank Accession LN492923 [GenBank]
Sequencing primer gene specific primer, JC24: 5'-GAACTTATCTTTCCGTTGAAGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K102589-0022-450-D01C-JC24
GCTCTTACTGAAGGTATTGGTATGCGTTAATCTGTGGCCCAAAAGCTGTTTCAAACTGTT
CTGCTGTTCCCGAGCCTCTCACGCTTCCACAAGATGGTCCAGGTATTAGGCATCTGCTTC
TCATAGCTCCCTATTCTTCTTCTGCGTCAATGTATCTCATCACTTCCATCTTTGGTTCTT
CTTTCTGTTTTAGATGAATCAGGAACTCGTACCAATGGCCATCACGTTGCTCTTGCATTT
GCCGCAAGCTTCAGTGCAGCATTTTTTGTTTTCTTTACAAGCGGAATGTTTCTTTGGTGG
AGATATCGCCGTAACAAGCAAATATTTTTTGACGTTAATGGTTAGTATCTTGAAATACTA
TCGGCTTCACATCATTTTGTGCAGAGCAAAACTGACACTTGAAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTC

Last Updated on 06.12.2018 16:11