Sequences from confirmation amplicon


Confirmation sequences for line 450H07 and hit MPO12 are shown.
Line ID 450H07
Locus MPO12 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16149616
Primers used for PCR CL96 - 8474, show primer details
   
Sequence Name 19-K119249-0022-450-H07C-CL96
GenBank Accession LN494985 [GenBank]
Sequencing primer gene specific primer, CL96: 5'-ATTGTTTTGGTGTTATTCGTGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K119249-0022-450-H07C-CL96
GAAACTAAAAAGTTCAAAATGCTTACATGACGAAACATATAATCTGAAAGATTGATTTCT
ATCACAGCCTAATTAATTTGATACTTATATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
AAACCTTTTTTTTGGCCCCCCCCTTTTTTATTTTTTTAAAAAAGGGTTAAAAAAAATTTT
TTTCCCAAAAAACCCCCTTGCCGTTTGGGAAATGGGGGAAAACCCCCCATTTCCAAATTT
TTAAAAACCCCCCGGGAAAAAAAATTCCAAAAAAAACCCCCTTTGGGGTCCCCCCCTGGG
GGGGACCAAAAATGGGGGCCCTGGTTGGTTTTTTAAAAACAAATTTTCAAACCCCCGGGG
GGGGGCCCCCCCCTTTTTTTGGGGGGAAAACCTTCCCCCCCCCCAAAAAAAAAAAAAGGG
GTTTCTTTCCGGGGAAACCCCCGGGGACCCCCAAGGGTTTGGGGGGCCAATTGGGAAAAA
AAAAAAAAAAATTCCCCCTTTTTTTTTCCTTCCAAAAGGGGGG
   
Sequence Name 03-K119248-0022-450-H07C-8409
GenBank Accession LN487703 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K119248-0022-450-H07C-8409
ATTTTCTTTTTCTCTGGTGTTCGTACAGCTCTTACCTCAATCATATGCTTGGGCACTCAA
CCGGTGATTATGAAATCTGATTGTCAAATATCAATCAAGGCCATCAATTTACGATCACCC
AATGTAGAGATCCATATATGGTATTTTATCTGATATTTTCTTACTTGCTAGTTCTCTAAA
CTTTTGTAATTGTACGTTTGTCCCATATCTCCAAACTGCATAGCTGATTTTCTGGCAAAA
ACTATTCTCTAAACATTGTTTAAATGATATAAGATATGGATTGACAAAAAATAAGTATAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTTTTTTCCAAATTTTTTGGGGGGGAAAA
AAAAAAACTTTTCAAAAAAAATTTTTCCCCCGGGAAACCTTTTTAAAATTTTTTTTTTTT
CAAATTTCCCCCATTTTTTTGGAAATTTTTGGAAAATTGGCCCCAAAAAACCCCAAAAAA
AAAA

Last Updated on 06.12.2018 16:11