Sequences from confirmation amplicon


Confirmation sequences for line 451A12 and hit At1g02110 are shown.
Line ID 451A12
Locus At1g02110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:395528
Primers used for PCR 66rq - 8474, show primer details
   
Sequence Name 31-K116272-0022-451-A12C-66rq
GenBank Accession LN500305 [GenBank]
Sequencing primer gene specific primer, 66rq: 5'-CAGAGAGAGGTGAGTCAACATCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K116272-0022-451-A12C-66rq
CTTGTGGCATTCGAGCTTCTGTCGCATCATTAAATTCCAGAGGGAGTTCATATGCTCTCT
CCACGCATGGTTCAAGCTGAGCCTGGTTCCCCTGAGCAACGGAGACCCAAAGAAACAGCG
GCCAGACTCATTTGCCTTGTGCGAGGAGTGGAAGCAGAGCCTGGAACGGGTGCCTGACAC
AGTGGCGTCAGAAGCCATAAAGAGCTTTGTAAACGTGGTACATGTGATATCAATAAAGCA
GGCGGAAGAGGTGAAGATGAAGAAACGCACGGAGAGTGCAGGAAAGGAGCTGGAGAAGAA
AGCATCCTCACTGAGGAGCATAGAGAGGAAGTACTACCAGGCATACTCGACGGTTGGGAT
AGGCCCTGGACCGGAGGTGTTGGACTCACGGGACCCGCTATCTGAGAAGAAATGTGAGCT
GGCGGCATGTCAGAGGCAGGTGGAGGATGAGGTAATGAGGCACGTGAAGGCTGTGGAGGT
GACACGAGCTATGACTCTCAACAATCTACAAACCGGCCTGCCCAATGTATTCCAGGCCTT
GACCAGCTTCTCATCTCTCTTCACTGAATCTCTCCAGACTGTCTGTTCTCGTTCCTACTC
CATCAACTGATTATGTCCAAGTTTCTCATTTATTTTTAAGCTCTCATTACGTTGGTATCA
TGTAAATTTGAGGATTGATTAAATTGAGTCTTGTGGTTTTGTGAATATGGATCAGACACG
AAGACATGAACCGAAGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
   
Sequence Name 07-K116271-0022-451-A12C-8409
GenBank Accession LN489429 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K116271-0022-451-A12C-8409
ATTCACAAAACCACAAGACTCAATTTAATCAATCCTCAAATTTACATGATACCAACGTAA
TGAGAGCTTAAAAATAAATGAGAAACTTGGACATAATCAGTTGATGGAGTAGGAACGAGA
ACAGACAGTCTGGAGAGATTCAGTGAAGAGAGATGAGAAGCTGGTCAAGGCCTGGAATAC
ATTGGGCAGGCCGGTTTGTAGATTGTTGAGAGTCATAGCTCGTGTCACCTCCACAGCCTT
CACGTGCCTCATTACCTCATCCTCCACCTGCCTCTGACATGCCGCCAGCTCACATTTCTT
CTCAGATAGCGGGTCCCGTGAGTCCAACACCTCCGGTCCAGGGCCTATCCCAACCGTCGA
GTATGCCTGGTAGTACTTCCTCTCTATGCTCCTCAGTGAGGATGCTTTCTTCTCCAGCTC
CTTTCCTGCACTCTCCGTGCGTTTCTTCATCTTCACCTCTTCCGCCTGCTTTATTGATAT
CACATGTACCACGTTTACAAAGCTCTTTATGGCTTCTGACGCCACTGTGTCAGGCACCCG
TTCCAGGCTCTGCTTCCACTCCTCGCACAAGGCAAATGAGTCTGGCCGCTGTTTCTTTGG
GTCTCCGTTGCTCAGGGGAACCAGGCTCAGCTTGAACCATGCGTGGAGAGAGCATATGAA
CTCCCTCTGGAATTTAATGATGCGACAGAAGCTCGAATGCCACAAGGACACAGCTGACTC
TAGGTCCCGCGTCACCTGCCGGTGTACCTCTG

Last Updated on 06.12.2018 16:11