Sequences from confirmation amplicon


Confirmation sequences for line 451B06 and hit At4g01100 are shown.
Line ID 451B06
Locus At4g01100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:477907
Primers used for PCR GL15 - 8474, show primer details
   
Sequence Name 27-K024724-022-451-B06C-8409
GenBank Accession LN498217 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K024724-022-451-B06C-8409
AATTTCCCGGACATGAAACTAGATAAGAGGAGCAGAAAAAACGCAGTCTTTACTCGATTA
GCATGTAAAAGAGTCACTTTTTCTTTCAGCCTTTGTGTTACATTCAATGCTCATTCTCAT
CGCTTAATGCGTGTTTCTATTAGGTCACGAACTGCTGTTGCACCTCTGGAAAGGATGAAG
ATATTACTTCAGGTTTTTTCCTTTGTTGATAGCTTTTGTTAAGGAACATATAAATGACTG
GAGAGTTAACCTGTTCGTTGTCAATCTCTAGGTCCAAAATCCGCATAATATAAAGTACAG
TGGGACAGTCCAAGGATTGAAGCATATTTGGAGAACCGAGGGACTTCGTGGACTGTTCAA
AGGAAATGGCACCAACTGTGCTCGTATTGTTCCTAATTCGGCTGTTAAATTTTTCAGCTA
TGAGCAAGCTTCAAAGTAAGACATCTATATCTTCTCTGTACTCTGTTATCTTGATCTTAA
TAAAGGCATCATACTTGATACTATAGATCTTTTTCTAATCTATGCTTTTTCTCTTTTTTC
TCTCATAGTGGCATACTGTACATGTATCGTCAGCGGACCGGAAATGGTAAATTGATTTCA
GCATAACATTTTTATTATTACCTGTTGAAGAATCTGTGCATGGTATTATTACCT
   
Sequence Name 28-K024724-022-451-B06C-GL15
GenBank Accession LN498684 [GenBank]
Sequencing primer gene specific primer, GL15: 5'-TTTAAAGGCTGAAGCGATAGGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K024724-022-451-B06C-GL15
GCACAGATTCTTCAACAGGTAATAATAAAAATGTTATGCTGAAATCAATTTACCATTTCC
GGTCCGCTGACGATACATGTACAGTATGCCACTATGAGAGAAAAAAGAGAAAAAGCATAG
ATTAGAAAAAGATCTATAGTATCAAGTATGATGCCTTTATTAAGATCAAGATAACAGAGT
ACAGAGAAGATATAGATGTCTTACTTTGAAGCTTGCTCATAGCTGAAAAATTTAACAGCC
GAATTAGGAACAATACGAGCACAGTTGGTGCCATTTCCTTTGAACAGTCCACGAAGTCCC
TCGGTTCTCCAAATATGCTTCAATCCTTGGACTGTCCCACTGTACTTTATATTATGCGGA
TTTTGGACCTAGAGATTGACAACGAACAGGTTAACTCTCCAGTCATTTATATGTTCCTTA
ACAAAAGCTATCAACAAAGGAAAAAACCTGAAGTAATATCTTCATCCTTTCCAGAGGTGC
AACAGCAGTTCGTGACCTAATAGAAACACGCATTAAGCGATGAGAATGAGCATTGAATGT
AACACAAAGGCTGAAAGAAAAAGTGACTCTTTTACATGCTAATCGAGTAAAGACTGCGTT
TTTCTGCTCCTCTTATCTAGTTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGA

Last Updated on 06.12.2018 16:11