Sequences from confirmation amplicon


Confirmation sequences for line 451C08 and hit At2g35260 are shown.
Line ID 451C08
Locus At2g35260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14851134
Primers used for PCR 09bq - 8474, show primer details
   
Sequence Name 55-K107839-0022-451-C08C-09bq
GenBank Accession LN508834 [GenBank]
Sequencing primer gene specific primer, 09bq: 5'-CCCTAATCACTATACGACCCCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K107839-0022-451-C08C-09bq
CAATCCCCCCCCACCAATTAACCATCTATATTTGATTTAAATCAACTTATCCGTGGCCTC
CGATCTAATGTGTTCGATTCTCCGGCGTAACCTTCTCCGGTGATCGTGAATTTTCCACAG
CCCATGCCCTAATATGACCGCCGAGTATATACCATGCGTCATCATCGGTGCTAATATATT
ATCCGTCTGAAAATTTGAATAAATTTTGAATTAATCAAGCGGTTTGGTTTTTCAGCAAAT
TATTGCATACCAAACCGAACAAAAACAAAAACCGAACCGAGAATTTACCTGAACCCATTC
AATACCGAGATAGAGGGCTAAGAGTCCTTCAAGGAGTGGAGAGTAAATCTTCTTCATCTG
TCTCTTCTCATACCAAGCTGCACAAAGCAAATGACAAAGAGGATTTCATGAGTAACAGAA
CAAACCGGTTTATATTTCCTTGTTCTCTAAGCTATTCTATTAGTTTATCTTTCATATTCT
TTCTCAAAACAAATATGATCAGACCAAGAAGAGAACAATTGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCACAGTTATT
   
Sequence Name 87-K107840-0022-451-C08C-8409
GenBank Accession LN514772 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K107840-0022-451-C08C-8409
GTCTGATCATATTTGTTTTGAGAAAGAATATGAAAGATAAACTAATAGAATAGCTTAGAG
AACAAGGAAATATAAACCGGTTTGTTCTGTTACTCATGAAATCCTCTTTGTCATTTGCTT
TGTGCAGCTTGGTATGAGAAGAGACAGATGAAGAAGATTTACTCTCCACTCCTTGAAGGA
CTCTTAGCCCTCTATCTCGGTATTGAATGGGTTCAGGTAAATTCTCGGTTCGGTTTTTGT
TTTTGTTCGGTTTGGTATGCAATAATTTGCTGAAAAACCAAACCGCTTGATTAATTCAAA
ATTTATTCAAATTTTCAGACGGATAATATATTAGCACCGATGATGACGCATGGTATATAC
TCGGCGGTCATATTAGGGCATGGGCTGTGGAAAATTCACGATCACCGGAGAAGGTTACGC
CGGAGAATCGAACACATTAGATCGGAGGCCACGGATAAGTTGATTTAAATCAAATATAGA
TGGTTTAATTGGTTGGGGGGTGGATTTGTGTGTATTTTTTTGNTATG

Last Updated on 06.12.2018 16:11