Sequences from confirmation amplicon


Confirmation sequences for line 452B06 and hit At4g38200 are shown.
Line ID 452B06
Locus At4g38200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17916404
Primers used for PCR GJ05 - 8474, show primer details
   
Sequence Name 39-K026770-022-452-B06C-8409
GenBank Accession LN503225 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K026770-022-452-B06C-8409
GTAGATTTCCCGGACATATAGCAACAAGCAGAGCAACTGCTTGATGGCATTTAGAAACCT
GCATATTTTTTGGGGAGGGAGAGTGAGCTCTTGGAGTTCATTACTACTGAGAGACGACTA
GACTAACCTGTGAAATGAAAGATAAAACAACTTGCCTTTCGTCAGAGAGCCAAATTGGGC
CGCCGTTATCAATGATGACTTTGAGCAACTCGAGAGACAAAGTCTTGCCTCTCACAAGAA
TCTGATCGTCGGTGTTCTCTTGTGACGAGAACTTCATAGACAACTTACAGAGATTTTTGA
AGAGAAGGAAACCGTCTTCCCTGATCTTGCTGCCTGTGCCTTCATCCTCAGTTGAAGAAG
CACCTTCCTCTGGTGGTTGGACTAGCGCGAAATCCGGAGGAGGAGCTGCTTCCCCGGCCG
TAATGACGTCGTTAATGAATCCCTGACAAATATGAACAGAGTTCCCTTCG
   
Sequence Name 40-K026770-022-452-B06C-GJ05
GenBank Accession LN503580 [GenBank]
Sequencing primer gene specific primer, GJ05: 5'-GATAAGAATGTCAACGAAGGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K026770-022-452-B06C-GJ05
GATTCATATTTGTCAGGGATTCATTAACGACGTCATTACGGCCGGGGAAGCAGCTCCTCC
TCCGGATTTCGCGCTAGTCCAACCACCAGAGGAAGGTGCTTCTTCAACTGAGGATGAAGG
CACAGGCAGCAAGATCAGGGAAGACGGTTTCCTTCTCTTCAAAAATCTCTGTAAGTTGTC
TATGAAGTTCTCGTCACAAGAGAACACCGACGATCAGATTCTTGTGAGAGGCAAGACTTT
GTCTCTCGAGTTGCTCAAAGTCATCATTGATAACGGCGGCCCAATTTGGCTCTCTGACGA
AAGGCAAGTTGTTTTATCTTTCATTTCACAGGTTAGTCTAGTCGTCTCTCAGTAGTAATG
AACTCCAAGAGCTCACTCTCCCTCCCCAAAAAATATGCAGGTTTCTAAATGCCATCAAGC
AGTTGCTCTGCTTGTTGCTATATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11