Sequences from confirmation amplicon


Confirmation sequences for line 452C09 and hit At5g50360 are shown.
Line ID 452C09
Locus At5g50360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20506013
Primers used for PCR 14nc - 8474, show primer details
   
Sequence Name 14-K117167-0022-452-C09C-14nc
GenBank Accession LN492686 [GenBank]
Sequencing primer gene specific primer, 14nc: 5'-CTAACATCATCTCTAATCCAACGG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117167-0022-452-C09C-14nc
AGCATCACATCAAGCCGCCACGCAAAGCCACCGTCACTCATCCTCCAAACATCACAGAAC
GAAGTAATCGAACTCACCTCCTTATCACGACACGTGTACTGCAATCTCATTCTACACGTA
AAAGTCCGGTACGTTACCAAAGGCTTCTCCTTCATCACAAGCCACACACTCATCTTACTA
CACTGCTCATGATCTTTCCCAACCACCAACTCTCTGTAAGAACCGTTCGTCCAAATGACT
CTCCCTAAACCGTCCGATATAAAACCAGGACACGTGTCTCTCTCAAGATTCATCTTCCTC
TCCTCGTCCGTACACCCTAACTCATACTCTCCCTCCATCAATCTCTCCGTTACACACTCC
ACCGTCAACAACGACGACACAACCGTTTCCGTCATTAACTCTGTCTGATAACGTCTGTTA
TAACTAGCTCCGTCATTAAAACTCAGCCACAACGCCGCCGCACCTAACTCCGGCGCCGCC
TTAGCCTTCAACGGCGAGTCTTTCTTCTCAGGAGTCTCTGGAAGAAGAGGCAACGTCACG
ATCTCTCTACCACCACCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATATCCGCAC
   
Sequence Name 03-K117166-0022-452-C09C-8409
GenBank Accession LN487655 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117166-0022-452-C09C-8409
GCATTTCATTGAATATATCCTGGTGGTGGTAGAGAGATCGTGACGTTGCCTCTTCTTCCA
GAGACTCCTGAGAAGAAAGACTCGCCGTTGAAGGCTAAGGCGGCGCCGGAGTTAGGTGCG
GCGGCGTTGTGGCTGAGTTTTAATGACGGAGCTAGTTATAACAGACGTTATCAGACAGAG
TTAATGACGGAAACGGTTGTGTCGTCGTTGTTGACGGTGGAGTGTGTAACGGAGAGATTG
ATGGAGGGAGAGTATGAGTTAGGGTGTACGGACGAGGAGAGGAAGATGAATCTTGAGAGA
GACACGTGTCCTGGTTTTATATCGGACGGTTTAGGGAGAGTCATTTGGACGAACGGTTCT
TACAGAGAGTTGGTGGTTGGGAAAGATCATGAGCAGTGTAGTAAGATGAGTGTGTGGCTT
GTGATGAAGGAGAAACCTTTGGTAACGTACCGGACTTTTACGTGTAGAATGAGATTGCAG
TACACGTGTCGTGATAAGGAGGTGAGTTCGATTACTTCGTTCTGTGATGTTTGGAGGATG
ATTGACGGAGGCTTTGCGTGGCGGCTTGATGTTGATGCTGCATTTTGCCTTGGACGGTGA
GGATTGGGC

Last Updated on 06.12.2018 16:11