Sequences from confirmation amplicon


Confirmation sequences for line 452D03 and hit At4g13430 are shown.
Line ID 452D03
Locus At4g13430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7807832
Primers used for PCR SR18 - 8474, show primer details
   
Sequence Name 48-K103544-0022-452-D03C-SR18
GenBank Accession LN506481 [GenBank]
Sequencing primer gene specific primer, SR18: 5'-CACTAATGACTTCTCCGAAGCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K103544-0022-452-D03C-SR18
TTTCCCTGATAAAATCCAACACACAAAACCATTGAAGTAAAATCAAACGAGAGAGAAAGA
AGAAGAAGAAGAAGAAGCTTATACCGATCCTGTAGTTCCAGGAGACCGATCCTTTTGAGG
AGCCATAACGGAAACAATCTTCCGTGAGATCGATTTCTTCTGACATCGATGAATCGAAAT
CTGAGAAGATTTAGGAAACGAAGAAATCCCCAAATCACTCTGCGAAAATCGACAAAACCT
ATAAGAAATTGAGAAAATGTGAAATCGAGAGAGAAAAGAAATGAAGGATAGATGAATAAC
CTTGGAGGATGATTTGCATAGAAAAGGAGAGGAAGAGATAACAGAAGCCATTGATTCTTC
TTGGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGC
   
Sequence Name 64-K103545-0022-452-D03C-8409
GenBank Accession LN511180 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K103545-0022-452-D03C-8409
GCCATTTACAATTGAATATATCCTCCAGAAGAATCAATGGCTTCTGTTATCTCTTCCTCT
CCTTTTCTATGCAAATCATCCTCCAAGGTTATTCATCTATCCTTCATTTCTTTTCTCTCT
CGATTTCACATTTTCTCAATTTCTTATAGGTTTTGTCGATTTTCGCAGAGTGATTTGGGG
ATTTCTTCGTTTCCTAAATCTTCTCAGATTTCGATTCATCGATGTCAGAAGAAATCGATC
TCACGGAAGATTGTTTCCGTTATGGCTCCTCAAAAGGATCGGTCTCCTGGAACTACAGGA
TCGGTATAAGCTTCTTCTTCTTCTTCTTCTTTCTCTCTCGTTTGATTTTACTTCAATGGT
TTTGTGTGTTGGATTTTATCAGGTGAAAACTGGGATGACAATGACGGAGAAGATTCTAGC
TAGAGCTTCGGAGAAGTCATTAGTG
Primers used for PCR SR19 - 8474, show primer details
   
Sequence Name 13-K103729-0022-452-D03C-SR19
GenBank Accession LN492007 [GenBank]
Sequencing primer gene specific primer, SR19: 5'-AGAAATAGTCCCAAATAGCAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K103729-0022-452-D03C-SR19
AATTCAAAATAGAGACACAAACTTAACAAAGCTTGATACAATGAAACCTCAATGAATTAA
TACTCGATATACTAATACCTTAAAATATTTTTTCTAGTTCTAAATTAATAATTTAACCTA
AAAATATCACTTCTATAAATTAATAATTACGATAATTTAATGAAATTTAGTAAACCATTA
ATCTCAATATTCTTAATTTATAGAGGTTTTACTAAATTGTAGAAACAACTAATTCGAGTA
CATCCCTGAATTAATAAATTTTAGAAATGTGAATTAACGAATACTTTTGTTCTTGTGAAT
GGTTAAAAAAAGTTACTTATCAAGACAAGTATGAAGTATCACGTGATTAAACGTTTAATG
ACACCAACCTAATGACAATTTGTTTGATTTATTTGTCACCTAACTAGAGACTCTCTCACA
GTCAACGCAGCTTATGTGTCATAGTAAGACTTTTTGTCTACTATAGTAGAAAGACGAATT
TATAACCCCTTTAGGTTTTTTCTAACACACGCCTCTNATCTCCGCGCACATGTAAATGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 29-K103730-0022-452-D03C-8409
GenBank Accession LN499253 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K103730-0022-452-D03C-8409
GCCATTTACTTTACATGTGCGCGGAGATTAGAGGCGTGTGTTAGAAAAAACCTAAAGGGG
TTATAAATTCGTCTTTCTACTATAGTAGACAAAAAGTCTTACTATGACACATAAGCTGCG
TTGACTGTGAGAGAGTCTCTAGTTAGGTGACAAATAAATCAAACAAATTGTCATTAGGTT
GGTGTCATTAAACGTTTAATCACGTGATACTTCATACTTGTCTTGATAAGTAACTTTTTT
TAACCATTCACAAGAACAAAAGTATTCGTTAATTCACATTTCTAAAATTTATTAATTCAG
GGATGTACTCGAATTAGTTGTTTCTACAATTTAGTAAAACCTCTATAAATTAAGAATATT
GAGATTAATGGTTTACTAAATTTCATTAAATTATCGTAATTATTAATTTATAGAAGTGAT
ATTTTTAGGTTAAATTATTAATTTAGAACTAGAAAAAATATTTTAAGGTATTAGTATATC
GAGTATTAATTCATTGAGGTTTCATTGTATCAAGCTTTGTTAAGTTTGTGTCTCTATTTT
AGAATTTGTTTGTGTTAATGTTTTAGTTTTGTTGCTTGCTATTT

Last Updated on 06.12.2018 16:11