Sequences from confirmation amplicon


Confirmation sequences for line 452E05 and hit At2g22950 are shown.
Line ID 452E05
Locus At2g22950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9766606
Primers used for PCR MK72 - 8474, show primer details
   
Sequence Name 46-K119006-0022-452-E05C-MK72
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, MK72: 5'-GCAATAGCAAAATACTCCAAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K119006-0022-452-E05C-MK72
GCCTAATGAAAGCTTCCTCATAAACATTCCTTGTACCAAAACCGCAAAGGTAACAATAGC
GAAGGAAAGACCAATTTTCCCAATGATGGTTGCAACTCCATTAAGTTTCACCTGCAACGG
AGTTTCGTCATCTCCTCCTTCACTAAGTGTTGCCATTAACTTTCCCCATTGAGTTCTCAT
CCCAACTGTTGTAACCAACATCTTACATGACCCATCTTGAACTTTGGTTCCAGAGAGAAG
GAAAGGGTTCTGTGCAGTCACCATCACAGGCTCACTCTCTCCAGTTAAACTCGATTCATC
GATAACAACAGAGAATCCCGAGAGGAAAAGACCATCTGCAGGGACTTGATCTCCGATAGC
AAGATGAACAACATCTCCAGGGAGCAAATCATATATAGACATCTTTTGTCTAAACCCGTT
TCGCGTAACTTGAACCGTGATCTTCTTCTTCTCTTTATCCAAATCCCGGAACTGCAAAGA
TTGTCTATAGTCACTAGTTGCTGTCACAAACACAACTAAAAGAATACTAGCAACAATGCC
AAGACCATCATGCGATCCTTGAGGCCATCCTTCAGTTGCAATCCCAACAATCAAAGAGAC
GAAAGCACAAACACCAAGAATCATAAGAGTCATATCTTGAAGTGCTTCCCAAACAAACAC
CCAGAAACTTCGTAATTCACTCTCTGCAAACTTATTGATTCCGAAAAGCTCTTGTCGTTT
GCTTAACTGCTCAGGTTCACCTGTTGAGAGACCAGCATTGGGACATGCCTTGAGCTTACC
TGAAAGACCATCAACACCACCATGGGACTTGAGCTTCTTCACATCATGACCTTCCACTAA
TGATCCTAACTCGTCTGCACAAATGTCAAAGCCTGCTGCTTTAACTTCCCTCAGCACACC
TAAAAA
   
Sequence Name 16-K119005-0022-452-E05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K119005-0022-452-E05C-8409
ATTTACATTGATCATTGTTTTTTAGGTGTGCCTGAGGAAGTTAAAGCAGCAGGCTTTGAC
ATTTGTGCAGACGAGTTAGGATCAATAGTGGAAGGTCATGATGTGAAGAAGCTCAAGTTC
CATGGTGGTGTTGATGGTCTTTCAGGTAAGCTCAAGGCATGTCCCAATGCTGGTCTCTCA
ACAGGTGAACCTGAGCAGTTAAGCAAACGACAAGAGCTTTTCGGAATCAATAAGTTTGCA
GAGAGTGAATTACGAAGTTTCTGGGTGTTTGTTTGGGAAGCACTTCAAGATATGACTCTT
ATGATTCTTGGTGTTTGTGCTTTCGTCTCTTTGATTGTTGGGATTGCAACTGAAGGATGG
CCTCAAGGATCGCATGATGGTCTTGGCATTGTTGCTAGTATTCTTTTAGTTGTGTTTGTG
ACAGCAACTAGTGACTATAGACAATCTTTGCAGTTCCGGGATTTGGATAAAGAGAAGAAG
AAGATCACGGTTCAAGTTACGCGAAACGGGTTTAGACAAAAGATGTCTATATATGATTTG
CTCCCTGGAGATGTTGTTCATCTTGCTATCGGAGATCAAGTCCCTGCAGATGGTCTTTTC
CTCTCGGGATTCTCTGTTGTTATCGATGAATCGAGTTTAACTGGAGAGAGTGAGCCTGTG
ATGGTGACTGCACAGAACCCTTTCCTTCTCTCTGGAACCAAAGTTCAAGATGGGTCATGT
AAGATGTTGGTTACAACAGTTGGGATGAGAACTCAATGGGGAAAGTTAATGGCAACACTT

Last Updated on 06.12.2018 16:11