Sequences from confirmation amplicon


Confirmation sequences for line 453E01 and hit At5g10300 are shown.
Line ID 453E01
Locus At5g10300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3240645
Primers used for PCR 05gt - 8474, show primer details
   
Sequence Name 21-K112133-0022-453-E01C-05gt
GenBank Accession LN495724 [GenBank]
Sequencing primer gene specific primer, 05gt: 5'-GAGTTTATTGAAGATGGGACCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K112133-0022-453-E01C-05gt
AGGTATTGTTCCACAAATCTCTAAAATTTGTTACGCCAAAACAACAAAATCTATATTCAA
ACTATGTGCTAAAGTTTTCGAATATGAATCATCGTATGAGATGATTATTAATTAGTTTTG
GTGTGTGGTTACATGATGGTTACACTGTGAAGGATTACGAGCTGGCAAAAATGTTGCATA
GGCAAGGGTCATTTTTCACAGAGGATCTATCAAAGAAAGAAAAGTTTAGCGAGGAAGGAT
ATGGTTCGGTGCAACGAGTTTACGTAATGAGTAGTGAAGACAAAGCCATCCCCTGCGATT
TCATTCGTTGGATGATTGATAATTTCAACGTCTCGAAAGTCTACGAGATCGATGGCGGAG
ATCACATGGCTTCATGTAAATATTCAATTGTAAATGGACCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACGGGTTATT
   
Sequence Name 05-K112132-0022-453-E01C-8409
GenBank Accession LN488420 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K112132-0022-453-E01C-8409
CCATCGATCTCGTAGACTTTCGAGACGTTGAAATTATCAATCATCCAACGAATGAAATCG
CAGGGGATGGCTTTGTCTTCACTACTCATTACGTAAACTCGTTGCACCGAACCATATCCT
TCCTCGCTAAACTTTTCTTTCTTTGATAGATCCTCTGTGAAAAATGACCCTTGCCTATGC
AACATTTTTGCCAGCTCGTAATCCTTCACAGTGTAACCATCATGTAACCACACACCAAAA
CTAATTAATAATCATCTCATACGATGATTCATATTCGAAAACTTTAGCACATAGTTTGAA
TATAGATTTTGTTGTTTTGGCGTAACAAATTTTAGAGATTTGTGGGAACAATACCTCTAT
GGGACAATTTTGGTAAAGACGTGCCTTCATGAATTTTGGTCCCATCTTCAATAAACT

Last Updated on 06.12.2018 16:11