Sequences from confirmation amplicon


Confirmation sequences for line 454A07 and hit At1g18835 are shown.
Line ID 454A07
Locus At1g18835 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6496285
Primers used for PCR 43sm - 8474, show primer details
   
Sequence Name 65-K116549-0022-454-A07C-43sm
GenBank Accession LN511474 [GenBank]
Sequencing primer gene specific primer, 43sm: 5'-CACCAAAATTATCTACGGCTCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K116549-0022-454-A07C-43sm
CTTAAGACTTCTGGGTTTTTAAATAATCAAAAGCAAGATCCGCCAAAAAAAGGAAAAACA
AAAAAATTGTTCCACATATTGTAATCTTAAATATAGGTACACATAGATTTGAAGTATGTA
TCACATCAAGATTGAAAAATCATTCATCATATAGATCGGCGTTAATGTAAGTAGTTGAAT
ATACAACATTAACCATATACATATACATATAAAATACATCTCGATCTAATATAAATCAAT
AGATCAAATCCAAAGCAGGTTCCTTTTTTCTTTGTTTTCAGTTACCTTTCTTGGCTTTTC
TTCTTTTTTCCATCTTCGTTCATCAGTTGTTTGCATTCGGAGGAGAATACTCACAAACAA
CCTCCGTGTCAACTTCCCTCCGGTGAAAATTCCGGTGACAGCCACAGGCAGCACACGTCA
AAGCATCATCTCCGCCGCTTGCCATAAACTCCCGGCAACCGTCAACAGCGTAGCCGCCAA
TATTAGCGGCATGATTCTTCTGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 25-K116548-0022-454-A07C-8409
GenBank Accession LN497615 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K116548-0022-454-A07C-8409
CAGAAGAATCATGCCGCTAATATTGGCGGCTACGCTGTTGACGGTTGCCGGGAGTTTATG
GCAAGCGGCGGAGATGATGCTTTGACGTGTGCTGCCTGTGGCTGTCACCGGAATTTTCAC
CGGAGGGAAGTTGACACGGAGGTTGTTTGTGAGTATTCTCCTCCGAATGCAAACAACTGA
TGAACGAAGATGGAAAAAAGAAGAAAAGCCAAGAAAGGTAACTGAAAACAAAGAAAAAAG
GAACCTGCTTTGGATTTGATCTATTGATTTATATTAGATCGAGATGTATTTTATATGTAT
ATGTATATGGTTAATGTTGTATATTCAACTACTTACATTAACGCCGATCTATATGATGAA
TGATTTTTCAATCTTGATGTGATACATACTTCAAATCTATGTGTACCTATATTTAAGATT
ACAATATGTGGAACAATTTTTTTGTTTTTCCTTTTTTTGGCGGATCTTGCTTTTGATTAT
TTTAAAAACCCAGAAGTCTTAAAGCGTTTAAGAAGTTGTCGATTTAGAATAGAATGAGCC
GTA

Last Updated on 06.12.2018 16:11